Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs
Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs
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DOI：
10.1016/j.bbrc.2008.02.111
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发表时间：
2008-05-09
影响因子：
3.1
通讯作者：
Iijima, Shinji
中科院分区：
文献类型：
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作者：
Ando, Munetoshi;Tu, Wenjie;Iijima, Shinji
我们检查了Siglec-9是否调节巨噬细胞系RAW 264中的细胞因子产生。表达Siglec-9的细胞在用脂多糖、肽聚糖、未甲基化CpG DNA和双链RNA刺激后产生低水平的肿瘤坏死因子（TNF）-α。另一方面，在表达Siglec-9的细胞中，白细胞介素（IL）-10的产生强烈增强。Siglec-5也表现出类似的活性。然而，当Siglec-9的胞质尾区中的两个酪氨酸残基突变为苯丙氨酸时，IL-10的上调以及TNF-α的下调被废除。Siglec-9的膜近端ITIM突变体不增强IL-10产生，但部分抑制TNF-α产生，表明TNF-α和IL-10的不同调节机制。Siglec-9还增强人巨噬细胞系THP-1中IL-10的产生。这些结果表明Siglec-9增强巨噬细胞中抗炎细胞因子IL-10的产生。(c)2008年爱思唯尔公司All rights reserved.
We examined whether Siglec-9 modulates cytokine production in the macrophage cell line RAW264. Cells expressing Siglec-9 produced low levels of tumor necrosis factor (TNF)-alpha upon stimulation with lipopolysaccharide, peptidoglycan, unmethylated CpG DNA, and double-stranded RNA. On the other hand, interleukin (IL)-10 production was strongly enhanced in Siglec-9-expressing cells. Similar activities were also exhibited by Siglec-5. However, the up-regulation of IL-10 as well as the down-regulation of TNF-alpha was abrogated when two tyrosine residues in the cytoplasmic tail of Siglec-9 were mutated to phenylalanine. A membrane proximal ITIM mutant of Siglec-9 did not enhance IL-10 production but partly inhibited TNF-alpha production, indicating diverse regulation mechanisms of TNF-alpha and IL-10. Siglec-9 also enhanced the production of IL-10 in the human macrophage cell line THP-1. These results demonstrate that Siglec-9 enhances the production of the anti-inflammatory cytokine IL-10 in macrophages. (c) 2008 Elsevier Inc. All rights reserved.