Association of Bone Mineral Density with Apolipoprotein E Phenotype
Association of Bone Mineral Density with Apolipoprotein E Phenotype
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骨矿物质密度与载脂蛋白 E 表型的关联
DOI：
10.1359/jbmr.1997.12.9.1438
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发表时间：
1997
影响因子：
6.2
通讯作者：
Y. Ouchi
中科院分区：
文献类型：
--
作者：
M. Shiraki;Y. Shiraki;C. Aoki;T. Hosoi;S. Inoue;M. Kaneki;Y. Ouchi
研究人员对 284 名年龄 47-82 岁（64.0 ± 1.0 岁，平均值 ± SE）的无关绝经后日本女性进行了载脂蛋白 E (Apo E) 表型及其与骨矿物质密度 (BMD) 的关系检查。通过等电聚焦方法分析 Apo E 表型，然后进行免疫印迹。还在同一人群中研究了 Apo E 表型与维生素 D 受体 (VDR) 基因或雌激素受体 (ER) 基因基因型之间的关系。我们人群中的 Apo E 表型频率为 E3/2 9.9%、E3/3 66.5%、E4/2 1.8%、E4/3 19.7% 和 E4/4 2.1%。我们将这些表型分为三类：Apo E4−/−（E3/2 和 E3/3，n = 217）、Apo E4+/-（E4/3 和 E4/2，n = 61）和 Apo E4+/+（E4/4，n = 6）。这三类人群的年龄、体重、身高和绝经年数没有显着差异。这三组的腰椎 BMD 值存在显着差异，依次为 E4−/− (0.91 ± 0.01 g/cm2)、E4+/- (0.85 ± 0.02 g/cm2) 和 E4++/+ (0.83 ± 0.06 g/cm2) (p = 0.031)。总 BMD 的 Z 分数也观察到相同的趋势 (p = 0.022)。 E4+/+ 中完整骨钙素的血清水平 (15.2 ± 5.7 ng/ml) 高于 E4−/− (7.7 ± 0.3 ng/ml) 或 E4+/- (7.7 ± 0.7 ng/ml)（方差分析 p = 0.004）。然而，骨转换标志物的血清或尿液水平没有其他显着差异。 E4+/+ 组的血清胆固醇往往高于其他两组 (p = 0.05)。 VDR 和 ER 基因型与 Apo E4 表型没有显着相关性。多元线性回归分析显示 Apo E4 是腰椎 BMD Z 评分的重要、独立预测因子。 Apo E4等位基因对腰椎BMD Z评分（-0.493±0.152）的影响与VDR的AAB（-0.616±0.225）或ER的PPxx（-0.785±0.314）没有显着差异。总之，Apo E4 等位基因与绝经后日本人的低骨量有关。
The phenotypes of apolipoprotein E (Apo E) and their relationship with the bone mineral density (BMD) were examined in 284 unrelated postmenopausal Japanese women aged 47–82 years (64.0 ± 1.0 years, mean ± SE). The Apo E phenotype was analyzed by the isoelectric focusing method, followed by immunoblotting. The relationship between the Apo E phenotype and the vitamin D receptor (VDR) gene or estrogen receptor (ER) gene genotypes was also studied in the same population. The Apo E phenotypic frequencies in our population were 9.9% for E3/2, 66.5% for E3/3, 1.8% for E4/2, 19.7% for E4/3, and 2.1% for E4/4. We classified these phenotypes into three categories: Apo E4−/− (E3/2 and E3/3, n = 217), Apo E4+/− (E4/3 and E4/2, n = 61), and Apo E4+/+ (E4/4, n = 6). The age, body weight, body height, and years since menopause were not significantly different among these three categories. The lumbar BMD values in these three groups were significantly different in the order of E4−/− (0.91 ± 0.01 g/cm2), E4+/− (0.85 ± 0.02 g/cm2), and E4+/+ (0.83 ± 0.06 g/cm2) (p = 0.031). The same trend was also observed for the Z score of the total BMD (p = 0.022). The serum level of intact osteocalcin in E4+/+ (15.2 ± 5.7 ng/ml) was higher than in E4−/− (7.7 ± 0.3 ng/ml) or E4+/− (7.7 ± 0.7 ng/ml) (p = 0.004 by analysis of variance). However, there were no other significant differences in the serum or urinary levels of bone turnover markers. Serum cholesterol in the E4+/+ group tended to be higher than in the other two groups (p = 0.05). There were no significant associations of the VDR and ER genotypes with the Apo E4 phenotype. A multivariate linear regression analysis revealed Apo E4 to be a significant, independent predictor of the Z score of the lumbar BMD. The effect of the Apo E4 allele on the Z score of the lumbar BMD (−0.493 ± 0.152) was not significantly different from that in the AAB of VDR (−0.616 ± 0.225) or PPxx of ER (−0.785 ± 0.314). In conclusion, the Apo E4 allele is associated with a low bone mass in postmenopausal Japanese.
脱辅基蛋白 E 表型的简化等电聚焦/免疫印迹测定。
影响因子：
9.3
作者：
Kataoka,S;Paidi,M;Howard,BV
通讯作者：
Howard,BV
人类载脂蛋白的遗传学研究：XIV。
影响因子：
2.9
作者：
Kamboh,MI;Kelly,LJ;Ferrell,RE
通讯作者：
Ferrell,RE