Doses Lactobacillus reuteri depend on adhesive ability to modulate the intestinal immune response and metabolism in mice challenged with lipopolysaccharide.
Doses Lactobacillus reuteri depend on adhesive ability to modulate the intestinal immune response and metabolism in mice challenged with lipopolysaccharide.
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DOI：
10.1038/srep28332
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发表时间：
2016-06-21
影响因子：
4.6
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Gao K;Liu L;Dou X;Wang C;Liu J;Zhang W;Wang H
本研究的目的是评价具有高粘附能力和低粘附能力的罗伊氏乳杆菌ZJ 617和ZJ 615以及鼠李糖乳杆菌GG（Lactobacillus rhamnosus GG，LGG）对脂多糖（lipopolysaccharide，LPS）刺激的小鼠免疫应答和代谢的调节作用。C57 BL/6小鼠经口接种ZJ 617、ZJ 615或LGG（1 × 108 CFU/只）1周后，腹腔注射LPS（10 mg/kg）24 h。与LPS刺激组相比，ZJ 615、ZJ 617和LGG均能显著降低LPS刺激小鼠血清TNF-α水平。ZJ 615和LGG可显著下调LPS刺激小鼠回肠细胞因子和Toll样受体的mRNA水平，抑制MAPK和NF-κB信号通路的激活，而ZJ 617可上调抗炎细胞因子IL-10的mRNA水平。相关分析证实，粘附能力与小鼠回肠的免疫调节作用有关。LPS组与ZJ 617 + LPS组、LPS组与ZJ 615 + LPS组、ZJ 617 + LPS组与ZJ 615 + LPS组之间分别有24、7、10种代谢产物和10、9、8种主要代谢途径存在显著性差异（VIP > 1，P < 0.05）。结果表明，ZJ 617和ZJ 615都能调节LPS刺激小鼠的肠道免疫反应和代谢。
The objective of this study was to evaluate the modulatory effects of Lactobacillus reuteri ZJ617 and ZJ615, which have high and low adhesive abilities, respectively, and Lactobacillus rhamnosus GG (LGG) on immune responses and metabolism in mice stimulated with lipopolysaccharide (LPS). Six C57BL/6 mice per group were orally inoculated with ZJ617, ZJ615 or LGG for one week (1 × 108 CFU/mouse) and i.p. injected with LPS (10 mg/kg) for 24 h. Compared with the LPS stimulation group, ZJ615, ZJ617 and LGG significantly decreased TNF-α levels in the sera of mice stimulated by LPS. ZJ615 and LGG significantly down-regulated mRNA levels of cytokines and Toll-like receptors, and suppressed activation of MAPK and NF-κB signaling, while ZJ617 up-regulated anti-inflammatory cytokine IL-10 mRNA levels in the ilea of mice stimulated by LPS. Correlation analysis confirmed that adhesive ability is relative with the immunomodulation in the ilea of mice. There were 24, 7 and 10 metabolites and 10, 9 and 8 major metabolic pathways with significant differences (VIP > 1, P < 0.05) between the LPS and ZJ617 + LPS groups, the LPS and ZJ615 + LPS groups, and the ZJ617 + LPS and ZJ615 + LPS groups, respectively. The results indicated that both ZJ617 and ZJ615 could modulate the intestinal immune responses and metabolism in LPS-stimulated mice.