Fabrication and in vitro evaluation of the collagen/hyaluronic acid PEM coating crosslinked with functionalized RGD peptide on titanium
Fabrication and in vitro evaluation of the collagen/hyaluronic acid PEM coating crosslinked with functionalized RGD peptide on titanium
复制标题
钛上功能化 RGD 肽交联的胶原/透明质酸 PEM 涂层的制备和体外评价
DOI：
10.1016/j.actbio.2011.10.020
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发表时间：
2012-02-01
影响因子：
9.7
通讯作者：
Zhao, Shifang
中科院分区：
文献类型：
--
作者：
Huang, Ying;Luo, Qiaojie;Zhao, Shifang
利用生物分子对钛进行表面改性是近年来钛表面改性研究的热点之一。在这项研究中，一种新的策略已被用来构建一个稳定的和生物活性的涂层钛。为此，合成了透明质酸（HA）的衍生物，即HA-GRGDSPC-（SH）。然后通过胶原和HA-GRGDSPC-（SH）的交替沉积（5个组装循环）和随后的交联（通过将游离巯基转化为二硫键（RGD-CHC-Ti基团）），在Ti上制备含二硫键交联Arg-Gly-Asp（RGD）的胶原/透明质酸基膜（REM）涂层。对PEM涂层的组装工艺和涂层的理化性能进行了详细的表征。PEM涂层在磷酸盐缓冲溶液中的稳定性可以通过交联度来调节，而在谷胱甘肽存在下的降解行为则具有谷胱甘肽浓度依赖性。RGD-CHC-Ti组MC 3 T3-E1细胞的粘附和增殖能力明显增强。RGD-CHC-Ti组骨特异性基因表达上调，碱性磷酸酶活性和骨钙素生成增加，矿化面积增加。这些结果表明，本文采用的策略可以作为在Ti上构建稳定的含RGD的生物活性涂层的有效方式。(C)2011 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Surface modification of titanium (Ti) using biomolecules has attracted much attention recently. In this study, a new strategy has been employed to construct a stable and bioactive coating on Ti. To this end, a derivative of hyaluronic acid (HA), i.e. HA-GRGDSPC-(SH), was synthesized. The disulfide-crosslinked Arg-Gly-Asp (RGD)-containing collagen/hyaluronic acid polyelectrolyte membrane (REM) coating was then fabricated on Ti through the alternate deposition of collagen and HA-GRGDSPC-(SH) with five assembly cycles and subsequent crosslinking via converting free sulphydryl groups into disulfide linkages (RGD-CHC-Ti group). The assembly processes for PEM coating and the physicochemical properties of the coating were carefully characterized. The stability of PEM coating in phosphate-buffered saline solution could be adjusted by the crosslinking degree, while its degradation behaviors in the presence of glutathione were glutathione concentration dependent. The adhesion and proliferation of MC3T3-E1 cells were significantly enhanced in the RGD-CHC-Ti group. Up-regulated bone specific genes, enhanced alkaline phosphatase activity and osteocalcin production, the increased areas of mineralization were also observed in the RGD-CHC-Ti group. These results indicate that the strategy employed herein may function as an effective way to construct stable, RGD-containing bioactive coatings on Ti. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.