Novel features of Drosophila period transcription revealed by real-time luciferase reporting
Novel features of Drosophila period transcription revealed by real-time luciferase reporting
复制标题
DOI：
10.1016/s0896-6273(00)80088-4
复制
发表时间：
1996-04-01
期刊：
影响因子：
16.2
通讯作者：
Hall, JC
中科院分区：
文献类型：
--
作者：
Brandes, C;Plautz, JD;Hall, JC
荧光素酶的快速周转及其检测的敏感性和非侵入性使该报告基因在时间基因表达研究中具有独特的位置。为了确定果蝇时钟基因周期(PER)的体内调控模式，我们构建了携带荧光素酶的转基因菌株，该基因融合到PER基因的启动子区域。这使得我们能够在几天内监测活着的成年人头部起搏细胞的生物发光的昼夜节律。这些高时间分辨率的实验使单个动物的神经元转录水平得以准确测量。这项新技术揭示了PER转录的一个新特征，并为在一般时间生物学中极大地简化实验和在广泛的实验系统中研究时间调节转录打开了大门。
The rapid turnover of luciferase and the sensitive, noninvasive nature of its assay make this reporter gene uniquely situated for temporal gene expression studies. To determine the in vivo regulatory pattern of the Drosophila clock gene period (per), we generated transgenic strains carrying a luciferase cDNA fused to the promoter region of the per gene. This has allowed us to monitor circadian rhythms of bioluminescence from pacemaker cells within the head for several days in individual living adults. These high time-resolution experiments permitted neuronal per transcription to be accurately measured in single animals. This new technology has revealed a novel feature of per transcription and opens the door to vastly simplified experiments in general chronobiology and studies of temporally regulated transcription in a wide range of experimental systems.