CONTROL OF TYPE-D GABAERGIC NEURON DIFFERENTIATION BY C-ELEGANS UNC-30 HOMEODOMAIN PROTEIN
CONTROL OF TYPE-D GABAERGIC NEURON DIFFERENTIATION BY C-ELEGANS UNC-30 HOMEODOMAIN PROTEIN
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DOI：
10.1038/372780a0
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发表时间：
1994-12-22
期刊：
影响因子：
64.8
通讯作者：
HORVITZ, HR
中科院分区：
文献类型：
--
作者：
JIN, YS;HOSKINS, R;HORVITZ, HR
秀丽隐杆线虫基因unc-30是19种抑制性GABA能（γ-氨基丁酸分泌）D型运动神经元（控制运动）发育和功能所必需的（1-4）。在unc-30突变体中，D神经元缺乏GABA（2），并且在轴突寻路和突触连接方面存在缺陷（J.白色，个人交流）。我们在这里报告，unc-30编码的同源结构域蛋白，是目前在核中的D神经元在年轻的幼虫，其中形成的运动电路的高水平，并在老年动物的低水平。在6个非GABA能神经元中也存在着GABA-30蛋白，而在7个非D型GABA能神经元中则不存在。unc-30的异位表达在通常不是GABA能的细胞中诱导GABA表达。我们建议，unc-30的功能作为D型神经元内的转录调节，以控制其终端分化，unc-30是足够的，但不是所有的细胞类型，以诱导GABA的表达。
THE Caenorhabditis elegans gene unc-30 is required for the develop ment and functioning of the 19 inhibitory GABAergic (gamma-aminobutyric-acid-secreting) type D motor neurons, which control locomotion(1-4). In unc-30 mutants the D neurons lack GABA(2) and have defects in axonal pathfinding and synaptic connections (J. White, personal communication). We report here that unc-30 encodes a homeodomain protein that is present in the nuclei of the D neurons at high levels in young larvae, in which the motor circuitry is formed, and at low levels in older animals. The UNC-30 protein is also present in six non-GABAergic neurons and is absent from the seven non-D-type GABAergic neurons. Ectopic expression of unc-30 induced GABA expression in cells that are normally not GABAergic. We propose that unc-30 functions as a transcriptional regulator within the type D neurons to control their terminal differentiation and that unc-30 is sufficient in some but not all cell types to induce GABA expression.