Direct inhibition of cell surface ephrin-B2 by recombinant ephrin-B2/FC.
Direct inhibition of cell surface ephrin-B2 by recombinant ephrin-B2/FC.
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DOI：
10.1016/j.bbrc.2013.09.070
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发表时间：
2013-10
影响因子：
3.1
通讯作者：
H. Xiaodong;H. Zhen;S. Min;Cui Zhiming;Jin Hongyan;Zhang Chong;Tan Xuefeng;Jin Guohua
中科院分区：
文献类型：
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作者：
H. Xiaodong;H. Zhen;S. Min;Cui Zhiming;Jin Hongyan;Zhang Chong;Tan Xuefeng;Jin Guohua
第一信使和病毒转染是刺激细胞输出信号的两种最常见的方法，尽管它们的应用是有限的。我们研究了重组ephrin-B2/Fc诱导神经干细胞分化的机制，发现其作为配体，通过直接干扰抑制维持神经祖细胞状态的内源性ephrin-B2。我们的研究结果显示了ephrin- b2 /Fc在细胞内的运动，并表明它循环到质膜表面，揭示了一种可能的ephrin运输模式。我们的结果也为在未来的研究中使用人工受体来直接输入信号重建ephrin的细胞内结构域提供了概念证明。
First messengers and viral transfection are the two most common ways to stimulate cells for signal output, although their applications are limited. We investigated mechanisms of inducing neural stem cell differentiation using recombinant ephrin-B2/Fc and found that it acted as a ligand and inhibited endogenous ephrin-B2, which maintenance of the neural progenitor cell state, by direct interference. Our results showed the movement of ephrin-B2/Fc within the cell and indicated that it recycled to the plasma membrane surface, revealing a possible pattern of ephrin trafficking. Our results also serve as proof of concept for the reconstruction of the intracellular domain of ephrin using an artificial receptor to direct input signals in future studies.