Interleukin-6 induces monocyte chemotactic protein-1 in peripheral blood mononuclear cells and in the U937 cell line
Interleukin-6 induces monocyte chemotactic protein-1 in peripheral blood mononuclear cells and in the U937 cell line
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DOI：
10.1182/blood.v91.1.258.258_258_265
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发表时间：
1998-01-01
期刊：
影响因子：
20.3
通讯作者：
Poli, G
中科院分区：
文献类型：
--
作者：
Biswas, P;Delfanti, F;Poli, G
促炎细胞因子刺激外周血单个核细胞（PBMC）诱导趋化因子基因表达在创伤修复和对感染因子的应答中起重要作用。在本研究中，我们发现，促炎细胞因子白细胞介素-8（IL-6）强效诱导PBMC中CC趋化因子单核细胞趋化蛋白1（MCP-1）的mRNA表达和分泌。此外，由于体内和体外人类免疫缺陷病毒（HIV）感染已显示出细胞因子的产生和/或对细胞因子的应答失调，因此研究了来自健康未感染和HIV感染个体的PBMC的MCP-1的组成性表达和IL-6诱导的MCP-1表达，在两组个体之间未观察到实质性差异。此外，IL-6上调单核细胞系U937及其慢性HIV感染对应物U1中MCP-1的表达。在这些细胞系中，IL-6选择性诱导MCP-1而不是其他趋化因子，包括活化后调节的正常T细胞表达和分泌（RANTES）、巨噬细胞炎性蛋白-1 α（MIP-1 α）、MIP-1 β和IL-8。IL-6对MCP-1的诱导作用可被氢化可的松部分抑制。因此，IL-6激活PBMC以分泌MCP-1，MCP-1是一种CC趋化因子，其对于发生重要炎症事件的组织和器官中的单核细胞募集至关重要，（C）1998，美国血液学会。
Induction of chemokine gene expression from peripheral blood mononuclear cells (PBMCs) stimulated by proinflammatory cytokines plays an important role in both wound repair and response to infectious agents. In the present study, we show that the proinflammatory cytokine interleukin-8 (IL-6) potently induced mRNA expression and secretion of the CC chemokine monocyte chemotactic protein 1 (MCP-1) in PBMCs. In addition, because human immunodeficiency virus (HIV) infection in vivo and in vitro has been shown to dysregulate the production of and/or the response to cytokines, PBMCs from both healthy uninfected and HIV-infected individuals were studied for their constitutive and IL-6-induced expression of MCP-1, No substantial differences were observed between the two groups of individuals. In addition, IL-6 upregulated MCP-1 expression in the promonocytic cell line U937 and in its chronically HIV-infected counterpart, U1. In these cell lines, IL-6 selectively induced MCP-1 and not other chemokines, including regulated upon activation normal T cells expressed and secreted (RANTES), macrophage inflammatory protein-1 alpha (MIP-1 alpha), MIP-1 beta, and IL-8. IL-6 induction of MCP-1 was partially inhibited by hydrocortisone in U1 cells. Thus, IL-6 activates PBMCs to secrete MCP-1, a CC chemokine pivotal for monocyte recruitment in tissue and organs in which important inflammatory events occur, (C) 1998 by The American Society of Hematology.