Green tea induces annexin-I expression in human lung adenocarcinoma A549 cells: involvement of annexin-I in actin remodeling
Green tea induces annexin-I expression in human lung adenocarcinoma A549 cells: involvement of annexin-I in actin remodeling
复制标题
DOI：
10.1038/labinvest.3700534
复制
发表时间：
2007-05-01
影响因子：
5
通讯作者：
Rao, Jian Yu
中科院分区：
文献类型：
--
作者：
Lu, Qing-Yi;Jin, Yu Sheng;Rao, Jian Yu
绿色茶多酚在多种体内外肿瘤模型中表现出多种抗肿瘤活性，其作用机制尚不清楚。以前，我们发现绿色茶提取物（GTE）调节肌动蛋白重塑在不同的细胞培养系统。肌动蛋白重构在癌细胞形态、细胞粘附、运动和侵袭中起重要作用。使用蛋白质组学方法，我们发现GTE诱导的膜联蛋白-I，一个多功能的肌动蛋白结合蛋白，在这些细胞系的表达。在这项研究中，我们的目的是进一步确定功能的作用，GTE诱导的膜联蛋白-I的表达在肌动蛋白重塑，细胞粘附和运动在肺腺癌A549细胞。我们发现GTE以剂量依赖性方式刺激膜联蛋白-I的表达。GTE诱导的膜联蛋白-I的表达似乎是在转录水平上，膜联蛋白-I的表达增加介导肌动蛋白聚合，导致细胞粘附增强和运动性降低。Annexin-I特异性干扰导致GTE诱导的肌动蛋白聚合和细胞粘附的损失，但不运动。事实上，膜联蛋白-I特异性干扰本身抑制运动，即使没有GTE。总之，膜联蛋白-I在GTE诱导的肌动蛋白重塑中起着重要作用，并且它可以作为与绿色茶的抗癌活性相关的潜在分子靶点。
Green tea polyphenols exhibit multiple antitumor activities in various in vitro and in vivo tumor models, and the mechanisms of action are not clear. Previously, we found that green tea extract ( GTE) regulates actin remodeling in different cell culture systems. Actin remodeling plays an important role in cancer cell morphology, cell adhesion, motility, and invasion. Using proteomic approaches, we found GTE-induced expression of annexin-I, a multifunctional actin binding protein, in these cell lines. In this study, we aimed to further define the functional role of GTE-induced annexin-I expression in actin remodeling, cell adhesion, and motility in lung adenocarcinoma A549 cells. We found that GTE stimulates the expression of annexin-I in a dose-dependent fashion. The GTE-induced annexin-I expression appears to be at the transcription level, and the increased annexin-I expression mediates actin polymerization, resulting in enhanced cell adhesion and decreased motility. Annexin-I specific interference resulted in loss of GTE-induced actin polymerization and cell adhesion, but not motility. In fact, annexin-I specific interference itself inhibited motility even without GTE. Together, annexin-I plays an important role in GTE-induced actin remodeling, and it may serve as a potential molecular target associated with the anticancer activities of green tea.