Bone regeneration of canine artificial alveolar clefts using bone-marrow-derived mesenchymal stromal cells and β-tricalcium phosphate: A preliminary study
Bone regeneration of canine artificial alveolar clefts using bone-marrow-derived mesenchymal stromal cells and β-tricalcium phosphate: A preliminary study
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DOI：
10.1016/j.odw.2012.01.003
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发表时间：
2012-06
影响因子：
0.4
通讯作者：
Y. Tokugawa;M. Kubota;Makoto Nishimura;N. Haruyama;K. Igarashi
中科院分区：
文献类型：
--
作者：
Y. Tokugawa;M. Kubota;Makoto Nishimura;N. Haruyama;K. Igarashi
唇腭裂是颌面部发病率最高的先天性疾病。本研究采用骨髓间充质干细胞（bone marrow derived mesenchymal stromal cells，MSCs）和β-磷酸三钙（β-tricalciumphosphate，β-TCP）复合物，对犬人工牙槽嵴裂进行骨再生。骨髓间充质干细胞/β-TCP组移植在β-TCP上培养的犬骨髓间充质干细胞。β-TCP组移植不含细胞的β-TCP。结果连续X线片显示，MSCs/β-TCP组β-TCP的吸收和骨替代进展顺利，再生骨与周围骨连续性良好。相反，在其他两组中，骨缺损在裂隙形成后13周仍然存在。MSCs/β-TCP组再生骨的骨密度介于正常骨和β-TCP组之间。MSCs/β-TCP组裂隙区骨矿含量是其他两组的3倍。组织学观察，MSCs/β-TCP组人工骨有骨替代。在对照组中，大部分的裂隙区充满了纤维组织。ConclusionsBone regeneration using MSCs and β-TCP presents a less-invasive new approach to alloy bone reconstruction.
ObjectiveCleft lip and palate is a congenital disease showing the highest incidence in the maxillofacial field. In this study, bone regeneration in the canine artificial alveolar clefts using bone-marrow-derived mesenchymal stromal cells (MSCs) and β-tricalcium phosphate (β-TCP) was attempted.MethodsArtificial clefts were prepared in 2-year-old dogs and the animals were divided into three groups. Canine MSCs cultured on β-TCP were grafted in the MSCs/β-TCP group. β-TCP without cells was grafted in the β-TCP group. Nothing was grafted in the control group.ResultsSerial X-ray photographs showed that absorption of β-TCP and substitution with bone progressed favorably and regenerated bone had smooth continuity with the surrounding bone in the MSCs/β-TCP group. In contrast, a bone defect remained at 13 weeks after the cleft formation in the other two groups. Bone mineral density of regenerated bone in the MSCs/β-TCP group was between the density of normal bone and that of β-TCP. Bone mineral content of the cleft region in the MSCs/β-TCP group was three times higher than that in the other two groups. Histologically, the artificial bone in the MSCs/β-TCP group showed replacement with bone. In the control group, most of the cleft region was filled with fibrous tissue.ConclusionsBone regeneration using MSCs and β-TCP presents the possibility of a less-invasive new approach to alveolar bone reconstruction.