Changes in the membrane fatty acid composition in Anoxybacillus flavithermus subsp. yunnanensis E13T as response to solvent stress
Changes in the membrane fatty acid composition in Anoxybacillus flavithermus subsp. yunnanensis E13T as response to solvent stress
复制标题
DOI：
10.1007/s00203-016-1266-9
复制
发表时间：
2016
影响因子：
2.8
通讯作者：
Hui Peng;L. Yi;Xiaohan Zhang;Yazhong Xiao;Yi Gao;Chao He
中科院分区：
文献类型：
--
作者：
Hui Peng;L. Yi;Xiaohan Zhang;Yazhong Xiao;Yi Gao;Chao He
黄热厌氧芽孢杆菌云南亚种是目前发现的第一种能够耐受多种溶剂的严格嗜热细菌。与大多数耐溶剂的嗜温细菌不同，该细菌不合成不饱和脂肪酸。结果表明，在A.黄热菌云南亚种E13 T、乙醇和甲苯导致直链脂肪酸（主要是C16：0）增加，从而导致更刚性的膜。此外，乙醇引起的直链脂肪酸的增加远高于甲苯。高温本身对脂肪酸组成的影响不大，而高温和乙醇的组合条件导致直链脂肪酸（主要是C16：0）的急剧增加，这是由减少支链脂肪酸平衡。增加也是温度依赖性的。60 ℃以上C16：0比例进一步增加。在其他四种缺氧杆菌中没有发现类似的证据。结果表明，A.与其嗜中温的对应物相比，云南黄热菌亚种似乎对溶剂产生不同的响应，其包括饱和直链/支链比率的增加。
Anoxybacillus flavithermussubsp.yunnanensisis currently the first species of strictly thermophilic bacteria that is able to tolerate a broad range of solvents. Unlike most of solvent-tolerant mesophilic bacteria, the bacterium does not synthesize unsaturated fatty acids. Our results revealed that in growing cells ofA. flavithermussubsp.yunnanensisE13T, ethanol and toluene resulted in an increase in straight-chain fatty acids, mainly C16:0, leading to a more rigid membrane. Moreover, the increase in straight-chain fatty acids caused by ethanol was much higher than that of toluene. High temperature had little effect on the fatty acid composition by itself, whereas the combined conditions of high temperature and ethanol caused the dramatic increase in straight-chain fatty acids (mainly C16:0), that was balanced by decreasing branched fatty acids. The increase was also temperature dependent. The proportion of C16:0 further increased above 60 °C. No similar evidence was found in four other species ofAnoxybacillus. The results suggested thatA. flavithermussubsp.yunnanesisseems to develop a different response to solvents compared to its mesophilic counterparts, which consist of an increase in the saturated straight/branched ratio.