Cre-loxP-Mediated Recombination: General Principles and Experimental Considerations.
Cre-loxP-Mediated Recombination: General Principles and Experimental Considerations.
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DOI：
10.1002/cpmo.22
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发表时间：
2017-03-02
影响因子：
--
通讯作者：
Pinto, Alexander R
中科院分区：
文献类型：
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作者：
McLellan, Micheal A;Rosenthal, Nadia A;Pinto, Alexander R
cre-loxP介导的重组系统（“cre-loxP系统”）是哺乳动物遗传学和细胞生物学的完整实验工具。该系统的使用极大地扩展了我们在小鼠中精确询问基因功能的能力，提供了基因表达的空间和时间控制。这在很大程度上是由于它的使用简单和它的适应性，以解决各种生物学问题。虽然cre-loxP系统的使用变得越来越广泛，特别是由于条件小鼠突变体的可用性不断增加，但在利用cre-loxP系统时需要考虑许多因素。本文综述了cre-loxP系统及其各种排列。它解决了cre-loxP技术的局限性和实验设计的相关考虑，并讨论了位点特异性遗传重组和整合的替代策略。John Wiley & Sons，Inc.
The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene expression. This has been largely due to the simplicity of its use and its adaptability to address diverse biological questions. While the use of the cre-loxP system is becoming increasingly widespread, in particular because of growing availability of conditional mouse mutants, many considerations need to be taken into account when utilizing the cre-loxP system. This review provides an overview of the cre-loxP system and its various permutations. It addresses the limitations of cre-loxP technology and related considerations for experimental design, and it discusses alternative strategies for site-specific genetic recombination and integration. © 2017 by John Wiley & Sons, Inc.