Mutations in VMK1, a mitogen-activated protein kinase gene, affect microsclerotia formation and pathogenicity in Verticillium dahliae
Mutations in VMK1, a mitogen-activated protein kinase gene, affect microsclerotia formation and pathogenicity in Verticillium dahliae
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DOI：
10.1007/s00294-005-0586-0
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发表时间：
2005-08-01
期刊：
影响因子：
2.5
通讯作者：
Dobinson, KF
中科院分区：
文献类型：
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作者：
Rauyaree, P;Ospina-Giraldo, MD;Dobinson, KF
大丽轮枝菌（Verticilliumdahliae）是一种重要的土传真菌病原菌，可引起多种重要农作物的枯萎病。由于其在土壤中的持久性，防治黄萎病在很大程度上依赖于土壤熏蒸。全球禁止甲基溴，一种高效的土壤熏蒸剂，提出了迫切需要开发替代控制措施，以防止黄萎病;这些可能是更好地理解的分子和细胞机制，支持V. dahliae的致病性。在这项研究中，我们评估了VMK 1在生长、发育和致病性中的作用，VMK 1是一种编码有丝分裂原活化蛋白（MAP）激酶（因此称为Verticillium MAP Kinase 1）的基因。通过根癌农杆菌介导的转化，在两个大丽轮枝菌分离物，一个来自生菜和其他番茄，VMK 1的中断，导致在不同的宿主植物中的毒力严重降低，这表明VMK 1是致病性和MAP激酶介导的信号通路在真菌致病性中具有保守的作用。vmk 1突变体也表现出减少分生孢子和微菌核形成，表明该基因是重要的多个细胞过程。
Verticillium dahliae is an important soil-borne fungal pathogen that causes vascular wilt diseases in a large variety of important crop plants. Due to its persistence in the soil, control of Verticilllum wilt relies heavily on soil fumigation. The global ban on methyl bromide, a highly effective soil fumigant, poses an urgent need to develop alternative control measures against Verticillium wilt; and these might be more forthcoming with a better understanding of the molecular and cellular mechanisms that underpin the pathogenicity of V. dahliae. In this study, we assessed the role in growth, development, and pathogenicity of VMK1, a gene encoding a mitogen-activated protein (MAP) kinase (hence, Verticillium MAP Kinase 1). Disruption of VMK1 via Agrobacterium tumefaciens-mediated transformation, in two V. dahliae isolates, one from lettuce and the other from tomato, resulted in severely reduced virulence in diverse host plants, suggesting that VMK1 is essential for pathogenicity and that the MAP kinase-mediated signaling pathway has a conserved role in fungal pathogenicity. The vmk1 mutants also exhibited reduced conidiation and microsclerotia formation, suggesting that the gene is important for multiple cellular processes.