Accuracy and Precision of Mini-FLOTAC and McMaster Techniques for Determining Equine Strongyle Egg Counts
Accuracy and Precision of Mini-FLOTAC and McMaster Techniques for Determining Equine Strongyle Egg Counts
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DOI：
10.1016/j.jevs.2016.09.006
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发表时间：
2017-01-01
影响因子：
1.3
通讯作者：
Nielsen, Martin K.
中科院分区：
文献类型：
--
作者：
Noel, Marie L.;Scare, Jessica A.;Nielsen, Martin K.
在寄生虫学研究和兽医实践中，使用粪卵计数技术间接评估肠道寄生虫负担和确定驱虫效果是很常见的。麦克马斯特方法是兽医实践中应用最广泛的技术之一，但最近，Mini-FLOTAC技术已被引入作为一种可能的替代方法。需要对这两种方法的精密度和准确度进行比较研究。本研究旨在评估Mini-FLOTAC技术通过两个部分的程序来确定马的粗线型卵数。首先，使用这两种方法执行了一组粪便计数。接下来，对添加的粪便样本进行盲法计数，真实鸡蛋计数为每克0、5、50、500和1,000个鸡蛋。所有计数都是一式三份，每个样本都使用两种方法进行计数。Mini-FLOTAC和McMaster的准确率分别为83.2%和53.7%。Mini-FLOTAC和McMaster的准确率分别为42.6%和23.5%。总之，这项研究发现Mini-FLOTAC显示出比McMaster技术更高的精密度和准确度，似乎是一种更可靠的替代技术。使用更精确的鸡蛋计数方法可以帮助确保治疗前后鸡蛋数量的变化反映出真正的减少，而不是由于机会的变化。(C)2016 Elsevier Inc.保留所有权利。
The use of fecal egg count techniques to indirectly assess intestinal parasite burdens and determine anthelmintic efficacy is common in parasitological research and veterinary practice. The McMaster method is one of the most widely used techniques in veterinary practice, but recently, the Mini-FLOTAC technique has been introduced as a possible alternative. Studies comparing the two methods in precision and accuracy are needed. This study aimed at evaluating the Mini-FLOTAC technique for determining equine strongyle egg counts through a two-part procedure. First, a set of fecal counts was executed using both methods. Next, blind counts were performed on spiked fecal samples with true egg counts at 0, 5, 50, 500, and 1,000 eggs per gram. All counts were performed in triplicates, and each sample was counted using both methods. Mini-FLOTAC and McMaster had 83.2% and 53.7% precision, respectively. The accuracy was found to be 42.6% and 23.5% for Mini-FLOTAC and McMaster, respectively. In conclusion, this study found that Mini-FLOTAC exhibited both higher precision and accuracy than the McMaster technique and appears to be a more reliable alternative. Using a more precise egg-counting method can help assure that changes in egg counts before and after treatment reflect a genuine reduction and are not due to chance variability. (C) 2016 Elsevier Inc. All rights reserved.