AN APHID-RESISTANCE LOCUS IS TIGHTLY LINKED TO THE NEMATODE-RESISTANCE GENE, MI, IN TOMATO
AN APHID-RESISTANCE LOCUS IS TIGHTLY LINKED TO THE NEMATODE-RESISTANCE GENE, MI, IN TOMATO
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DOI：
10.1073/pnas.92.2.622
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发表时间：
1995-01-17
影响因子：
11.1
通讯作者：
WILLIAMSON, VM
中科院分区：
文献类型：
--
作者：
KALOSHIAN, I;LANGE, WH;WILLIAMSON, VM
在加州戴维斯，不同育种项目的番茄品系对马铃薯蚜虫（Macrosiphum euphorbiae）自然侵染的抗性进行了田间评估。结果表明，所有携带抗线虫基因Mi的品系都表现出抗蚜能力。为了研究这种关系，我们开发了一种温室蚜虫抗性试验。线虫和蚜虫抗性在近等基因系中的关联表明，这些性状是紧密相连的。对一个F-2群体的线虫抗性分离分析表明，蚜虫抗性分离为与Mi遗传相关的单个主要位点，建议将该位点命名为Meu1。Meu1很可能是与野生种番茄中的Mi一起被引入番茄的。在Motelle系中存在抗蚜性，其中包含一个非常小的DNA渗入区域，并且缺乏重组，这表明Meu1与Mi紧密相连或可能是同一个基因，目前用于克隆Mi的基于图谱的策略应该适用于克隆Meu1。
Tomato lines from diverse breeding programs were evaluated in the field for resistance to a natural infestation of the potato aphid, Macrosiphum euphorbiae, in Davis, CA. It was noted that all lines that carried the nematode-resistance gene, Mi, displayed aphid resistance. A greenhouse assay for aphid resistance was developed to investigate this relationship. Association of nematode and aphid resistances in near-isogenic lines suggested that these traits are tightly linked. Analysis of an F-2 population segregating for nematode resistance indicated that aphid resistance segregated as a single major locus genetically linked to Mi. The name Meu1 is proposed for this locus. It is likely that Meu1 was introduced into tomato along with Mi from the wild species Lycopersicon peruvianum. The presence of aphid resistance in the line Motelle, which contains a very small region of introgressed DNA, and the lack of recombinants suggest that Meu1 is tightly linked to Mi or possibly is the same gene, The map-based strategy currently being used to clone Mi should be applicable to cloning Meu1.