A qualitative fluorescence-based assay for tyrosyl radical scavenging activity: ovothiol A is an efficient scavenger.
A qualitative fluorescence-based assay for tyrosyl radical scavenging activity: ovothiol A is an efficient scavenger.
复制标题
基于荧光的定性测定酪氨酰自由基清除活性：卵硫醇 A 是一种有效的清除剂。
DOI：
10.1016/0003-2697(89)90440-5
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发表时间：
1989
影响因子：
2.9
通讯作者：
Hopkins,PB
中科院分区：
文献类型：
--
作者：
Holler,TP;Hopkins,PB
本文介绍了一种测定抗氧化剂清除酪氨酰自由基活性的方法，该方法仅需标准荧光计和市售材料。含有超氧化物歧化酶和过氧化氢酶的酪氨酸水溶液的紫外线照射通过光生酪氨酰自由基的二聚化产生荧光二酪氨酸残基。添加的抗氧化剂通过清除酪氨酰基自由基来抑制荧光的积累。物质抑制二酪氨酸形成的能力与该物质的单电子氧化电位之间存在相关性。该方法表明，卵硫醇A比谷胱甘肽或半胱氨酸更有效地清除酪氨酰基自由基，类似于已知的生物自由基清除剂尿酸和抗坏血酸以及α-生育酚类似物trolox。
A method for determining relative tyrosyl radical scavenging activity of antioxidants which requires only a standard fluorometer and commercially available materials is presented. Ultraviolet irradiation of aqueous tyrosine solutions containing superoxide dismutase and catalase produces fluorescent dityrosine residues via dimerization of photogenerated tyrosyl radicals. Added antioxidants suppress the buildup of fluorescence by scavenging the tyrosyl radicals. A correlation exists between the ability of a substance to suppress dityrosine formation and the substance's one-electron oxidation potential. This method demonstrates that ovothiol A scavenges tyrosyl radicals much more efficiently than glutathione or cysteine, resembling instead the known biological radical scavengers uric acid and ascorbic acid and the α-tocopherol analog trolox.