Heterotrophic symbionts of phototrophic consortia: members of a novel diverse cluster of Betaproteobacteria characterized by a tandem rrn operon structure.
Heterotrophic symbionts of phototrophic consortia: members of a novel diverse cluster of Betaproteobacteria characterized by a tandem rrn operon structure.
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光养菌群的异养共生体：以串联 rrn 操纵子结构为特征的新型多样化 Betaproteobacteria 簇的成员
DOI：
10.1111/j.1462-2920.2007.01390.x
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发表时间：
2007
影响因子：
5.1
通讯作者：
Overmann
中科院分区：
文献类型：
--
作者：
Pfannes;Overmann
光养聚生体代表了细菌种间关联的最高度发达的类型，并且由附着在中心无色杆状细菌周围的绿色硫细菌附着生物组成。基于16 S rRNA基因测序，最近发现该菌群的中心细菌“Chlorochromatium aggregatum”代表了变形菌门β亚群中丛枝菌科的一个新的系统发育分离谱系。迄今为止，已根据16 S rRNA基因序列对19种不同类型的光合菌进行了分类，但异养菌的多样性和系统发育关系尚不清楚。我们开发了一种基于'C. aggregatum'中心细菌的特异性rrn（核糖体RNA）操纵子结构的方法，以从自然聚生群体中恢复其他中心细菌及其近亲的16 S rRNA基因序列。 首先通过采用单层生物膜中聚生体生长的选择性方法将“C. aggregatum”中心细菌的基因组DNA富集数百倍，然后通过氯化铯-双苯并咪唑平衡密度梯度离心纯化中心细菌的基因组。反向PCR，克隆和测序的组合显示，中央细菌的worrnoperons以串联的方式排列，并由195个碱基对的异常短的基因间区域分开。利用这种罕见的基因顺序，通过PCR筛选各种天然微生物群落。我们在淡水湖泊的化跃层中发现了一个多样的、以前未知的β-变形菌亚群。在其他淡水和土壤样品中没有这一组。所有回收的16 S rRNA基因序列均与聚集型C. aggregatum中心菌的16 S rRNA基因序列相关。 原位杂交结果表明，其中两个序列来源于不同光养菌群的中心细菌，然而，这两个序列仅与“C. aggregatum”的中心细菌有较远的亲缘关系。 基于详细的系统发育分析，这些中央细菌共生体的光合聚生体有一个多系起源。
Phototrophic consortia represent the most highly developed type of interspecific association of bacteria and consist of green sulfur bacterial epibionts attached around a central colourless rod‐shaped bacterium. Based on 16S rRNA gene sequencing, the central bacterium of the consortium ‘Chlorochromatium aggregatum’ was recently shown to represent a novel and phylogenetically isolated lineage of theComamonadaceaewithin the β‐subgroup of theProteobacteria. To date, 19 types of phototrophic consortia are distinguished based on the different 16S rRNA gene sequences of their epibionts, but the diversity and phylogenetic relationships of the heterotrophic partner bacteria are still unknown. We developed an approach based on the specificrrn(ribosomal RNA) operon structure of the central bacterium of ‘C. aggregatum’ to recover 16S rRNA gene sequences of other central bacteria and their close relatives from natural consortia populations. Genomic DNA of the central bacterium of ‘C. aggregatum’ was first enriched several hundred‐fold by employing a selective method for growth of consortia in a monolayer biofilm followed by a purification of the genome of the central bacterium by cesium chloride‐bisbenzimidazole equilibrium density gradient centrifugation. A combination of inverse PCR, cloning and sequencing revealed that tworrnoperons of the central bacterium are arranged in a tandem fashion and are separated by an unusually short intergenic region of 195 base pairs. This rare gene order was exploited to screen various natural microbial communities by PCR. We discovered a diverse and previously unknown subgroup ofBetaproteobacteriain the chemoclines of freshwater lakes. This group was absent in other freshwater and soil samples. All the 16S rRNA gene sequences recovered are related to that of the central bacterium of ‘C. aggregatum’. Fluorescencein situhybridization indicated that two of these sequences originated from central bacteria of different phototrophic consortia, which, however, were only distantly related to the central bacterium of ‘C. aggregatum’. Based on a detailed phylogenetic analysis, these central bacterial symbionts of phototrophic consortia have a polyphyletic origin.
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影响因子：
2.4
作者：
J. Overmann;M. Tilzer
通讯作者：
M. Tilzer
DOI：
--
发表时间：
1988
期刊：
影响因子：
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作者：
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作者：
A. Sass;H. Sass;M. Coolen;H. Cypionka;J. Overmann
通讯作者：
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DOI：
--
发表时间：
1995
期刊：
影响因子：
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作者：
J. Gasol;K. Jürgens;R. Massana;Juan I. Calderón;C. Pedrós
通讯作者：
C. Pedrós
影响因子：
4.4
作者：
Kanzler, BEM;Pfannes, KR;Overmann, J
通讯作者：
Overmann, J