In vitro fermentation of Pennisetum clandestinum Hochst. Ex Chiov increased methane production with ruminal fluid adapted to crude glycerol
In vitro fermentation of Pennisetum clandestinum Hochst. Ex Chiov increased methane production with ruminal fluid adapted to crude glycerol
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DOI：
10.1007/s11250-019-02043-5
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发表时间：
2020-03-01
影响因子：
1.7
通讯作者：
Gomez, Alejandra Marin
中科院分区：
文献类型：
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作者：
Echavarria, Diana Marcela Valencia;Valderrama, Luis Alfonso Giraldo;Gomez, Alejandra Marin
本试验的目的是确定适合于粗甘油补充的瘤胃液对牧草狼尾草（Pennisetum clandestinum）霍赫斯特体外甲烷和发酵参数的影响。Ex Chiov（基库尤饲料）35天（D35）和45天（D45）的再生。在随机拉丁方设计（4只动物x4个阶段）下，使用4头600 +/- 33 kg LW的非泌乳期插管荷斯坦奶牛，在每个阶段适应13天后获得瘤胃液，以在瘤胃中补充粗甘油（GL）：0（GL 0）、500（GL 1）、1000（GL 2）和1500 g/奶牛/天（GL 3）。所有变量均在4 x 2裂区布置下进行分析，其中分析了瘤胃液对补充GL的适应及其与孵育饮食（GLx D）的相互作用。瘤胃pH值和干物质（DMd）和有机物（OMd）的体外表观降解率不受影响，与NH3-N和乙酸的浓度下降，由适应粗甘油的瘤胃液。丙酸盐和丁酸盐的浓度增加，但不影响瘤胃液中VFA的总浓度。结果表明，D35、GL 1、GL 2、GL 3组的体外产甲烷量均高于对照组。D45饲料的体外甲烷产量随着适应GL 2和GL 3的瘤胃液的增加而增加。瘤胃液适应粗甘油补充增加体外甲烷生产时，孵化饲料基库尤。
The aim of this trial was to determine the effect of ruminal fluid adapted to crude glycerol supplementation on in vitro methane and fermentation parameters of the forage Pennisetum clandestinum Hochst. Ex Chiov (Kikuyu forage) of 35 (D35) and 45 days (D45) of regrowth. Four non-lactating cannulated Holstein cows with 600 +/- 33 kg LW, under a randomized Latin square design (4 animals x 4 periods), were used to obtain ruminal fluid after adaptation for 13 days within each period to supplementation in the rumen with crude glycerol (GL): 0 (GL0), 500 (GL1), 1000 (GL2), and 1500 g/cow/day (GL3). All the variables were analyzed under a 4 x 2 split-plot arrangement, where the ruminal fluid adaptation to supplementation with GL and its interaction with the incubated diets (GLx D) were analyzed. Ruminal pH values and in vitro apparent degradability of the dry matter (DMd) and organic matter (OMd) were not affected, with a decrease in the concentrations of NH3-N and acetate by the ruminal fluid adapted to crude glycerol. The concentrations of propionate and butyrate increased, without affecting the total concentration of VFA in the ruminal fluid. The in vitro methane production increased with forage D35 and GL1, GL2, GL3. The in vitro methane production with the D45 forage increased with the ruminal fluid adapted to GL2 and GL3. The ruminal fluid adapted to crude glycerol supplementation increased in vitro methane production when incubating the forage Kikuyu.