Salt-induced immobilization of affinity ligands onto epoxide-activated supports.
Salt-induced immobilization of affinity ligands onto epoxide-activated supports.
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DOI：
10.1016/s0021-9673(99)00484-7
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发表时间：
1999-07
期刊：
影响因子：
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通讯作者：
Jeffrey B. Wheatley;Donald E. Schmidt
中科院分区：
文献类型：
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作者：
Jeffrey B. Wheatley;Donald E. Schmidt
在高浓度的某些盐，如硫酸铵和磷酸钾，增强了环氧活化固定相上的亲和配体的固定。这种增强被认为是由于盐诱导的亲和配体和固定相表面之间的疏水相互作用而发生的。反应性环氧基团附近亲和配体浓度的增加导致亲和配体上的亲核基团与环氧化物之间的反应速率增加。盐诱导的增强适用于蛋白质和核苷酸在中性pH值和小的亲和配体在升高的pH值。在大多数情况下，环氧基团的水解不限制固定的亲和配体的量。这篇综述讨论了使用高盐浓度将蛋白质、寡核苷酸和肽固定在环氧活化的二氧化硅和聚合物载体上。这些修饰的支持物可用于亲和应用，如亲和色谱或免疫测定。
The immobilization of affinity ligands onto epoxy-activated stationary phases is enhanced at high concentrations of certain salts, such as ammonium sulfate and potassium phosphate. This enhancement is thought to occur because of a salt-induced hydrophobic interaction between the affinity ligand and the surface of the stationary phase. The increase in concentration of the affinity ligand near the reactive epoxy groups leads to an increase in the rate of reaction between the nucleophilic groups on the affinity ligand and the epoxide. The salt-induced enhancement is applicable to proteins and nucleotides at neutral pH and to small affinity ligands at elevated pH. In most cases, the hydrolysis of the epoxy groups does not limit the amount of affinity ligand immobilized. This review discusses the use of high salt concentrations to immobilize proteins, oligonucleotides and peptides to epoxy-activated silica and polymer supports. These modified supports can be used in affinity applications such as affinity chromatography or immunoassays.