Dicyanoisophorone-Based Near-Infrared-Emission Fluorescent Probe for Detecting NAD(P)H in Living Cells and in Vivo
Dicyanoisophorone-Based Near-Infrared-Emission Fluorescent Probe for Detecting NAD(P)H in Living Cells and in Vivo
复制标题
用于检测活细胞和体内 NAD(P)H 的基于二氰基异佛尔酮的近红外发射荧光探针
DOI：
10.1021/acs.analchem.8b03563
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发表时间：
2019
影响因子：
7.4
通讯作者：
Tang Bo
中科院分区：
文献类型：
--
作者：
Zhao Yuehui;Wei Keyan;Kong Fanpeng;Gao Xiaonan;Xu Kehua;Tang Bo
NADH和NADPH是所有活细胞中普遍存在的辅酶，在细胞能量代谢的许多氧化还原反应中起重要作用。准确检测生理条件下NAD（P）H的分布和动态变化，对于了解其生物学功能和病理作用至关重要。在这项工作中，我们开发了一种近红外（NIR）发射荧光小分子探针（DCI-MQ）组成的二氰基异佛尔酮生色团共轭喹啉部分在体内检测NAD（P）H。DCI-MQ具有高水溶性、快速响应、非凡的选择性、高灵敏度（检测限为12 nM）、低细胞毒性和响应NAD（P）H的NIR发射（660 nm）的优点。此外，DCI-MQ探针成功应用于活体细胞和荷瘤小鼠内源性NAD（P）H的检测和成像，为研究NAD（P）H相关的生理和病理过程提供了有效的工具。
NADH and NADPH are ubiquitous coenzymes in all living cells that play vital roles in numerous redox reactions in cellular energy metabolism. To accurately detect the distribution and dynamic changes of NAD(P)H under physiological conditions is essential for understanding their biological functions and pathological roles. In this work, we developed a near-infrared (NIR)-emission fluorescent small-molecule probe (DCI-MQ) composed of a dicyanoisophorone chromophore conjugated to a quinolinium moiety for in vivo NAD(P)H detection. DCI-MQ has the advantages of high water solubility, a rapid response, extraordinary selectivity, great sensitivity (a detection limit of 12 nM), low cytotoxicity, and NIR emission (660 nm) in response to NAD(P)H. Moreover, the probe DCI-MQ was successfully applied for the detection and imaging of endogenous NAD(P)H in both living cells and tumor-bearing mice, which provides an effective tool for the study of NAD(P)H-related physiological and pathological processes.