Mimetic peptides as blockers of connexin channel-facilitated intercellular communication
Mimetic peptides as blockers of connexin channel-facilitated intercellular communication
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DOI：
10.1080/15419060801891034
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发表时间：
2007-11-01
影响因子：
--
通讯作者：
Leybaert, Luc
中科院分区：
文献类型：
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作者：
Evans, W. Howard;Leybaert, Luc
缺乏连接蛋白介导的细胞间通讯的化学抑制剂。短的“设计师”连接蛋白模拟肽的出现提供了快速和可逆地抑制连接蛋白通道的新工具。该观点描述了模拟肽的发展，特别是Gap 26和27，它们是最流行的，并且对应于连接蛋白37、40和43的细胞外环中的特定序列。最初，它们被用于抑制广泛的哺乳动物细胞和组织中的缝隙连接偶联。目前，它们也被研究作为治疗剂，加速伤口愈合和脊髓损伤的早期治疗。模拟肽与连接蛋白半通道结合，影响通道性质，如通过降低电导率和有效阻断小报告染料的进入和细胞释放ATP所示。提出了一种机制来帮助解释连接蛋白模拟肽对连接蛋白半通道和间隙连接偶联的双重作用。
There is a dearth of chemical inhibitors of connexin-mediated intercellular communication. The advent of short "designer" connexin mimetic peptides has provided new tools to inhibit connexin channels quickly and reversibly. This perspective describes the development of mimetic peptides, especially Gap 26 and 27 that are the most popular and correspond to specific sequences in the extracellular loops of connexins 37,40 and 43. Initially they were used to inhibit gap-junctional coupling in a wide range of mammalian cells and tissues. Currently, they are also being examined as therapeutic agents that accelerate wound healing and ill the early treatment of spinal cord injury. The mimetic peptides bind to connexin hemichannels, influencing channel properties as shown by lowering of electrical conductivity and potently blocking the entry of small reporter dyes and the release of ATP by cells. A mechanism is proposed to help explain the dual action of connexin mimetic peptides on connexin hemichannels and gap-junctional coupling.