High Fat Diet Alters Gut Microbiota and the Expression of Paneth Cell-Antimicrobial Peptides Preceding Changes of Circulating Inflammatory Cytokines.
High Fat Diet Alters Gut Microbiota and the Expression of Paneth Cell-Antimicrobial Peptides Preceding Changes of Circulating Inflammatory Cytokines.
复制标题
高脂肪饮食改变肠道微生物群和潘氏细胞抗菌肽的表达，先于循环炎症细胞因子的变化
DOI：
10.1155/2017/9474896
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发表时间：
2017
影响因子：
4.6
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Guo X;Li J;Tang R;Zhang G;Zeng H;Wood RJ;Liu Z
肥胖是包括肠癌在内的许多疾病的既定风险因素。其中一个重要的机制是肥胖导致的慢性炎症。然而，饮食诱导的肥胖是通过脂肪组织中产生的细胞因子加剧肠道炎症状态，还是高脂饮食首先改变肠道微生物区系，然后驱动肠道炎症，这一点仍有待确定。为了解决这一问题，我们用高脂饲料(HF，60%)喂养C57BL/6小鼠，并在8、12和16周后依次处死它们，然后测定肠道微生物区系组成和抗菌肽的表达。肠道微生物区系组成在饲喂HF后8周发生改变，12周和16周时降低潘氏抗菌肽溶菌酶和Reg IIIγ(p<；0.05)，而循环炎性细胞因子干扰素γ和肿瘤坏死因子-α在饲喂16周时升高(p<；0.05)。这些结果表明，高脂饮食可能通过改变肠道微生物区系来刺激肠道炎症，并且先于循环炎症细胞因子的潜在影响而发生。这些发现强调了微生物区系的重要性，除了脂肪组织本身，在驱动肠道炎症，这可能会促进肠道肿瘤的发生。
Obesity is an established risk factor for many diseases including intestinal cancer. One of the responsible mechanisms is the chronic inflammation driven by obesity. However, it remains to be defined whether diet-induced obesity exacerbates the intestinal inflammatory status by cytokines produced in adipose tissue or the high fat diet first alters the gut microbiota and then drives intestinal inflammation. To address this question, we fed C57BL/6 mice with a high fat diet (HF, 60%) and sacrificed them sequentially after 8, 12, and 16 weeks, and then compositions of gut microbiota and expressions of antimicrobial peptides were determined. The compositions of gut microbiota were altered at 8 wk HF feeding, followed with reduced Paneth antimicrobial peptides lysozyme and Reg IIIγ after 12 and 16 wk HF feeding (p < 0.05), whereas elevations of circulating inflammatory cytokines IFNγ and TNF-α were observed until feeding a HF diet for 16 weeks (p < 0.05). These results indicated that high fat diet may stimulate intestinal inflammation via altering gut microbiota, and it occurs prior to the potential influence by circulating inflammatory cytokines. These findings emphasized the importance of microbiota, in addition to adipose tissue per se, in driving intestinal inflammation, which may thereafter promote intestinal tumorigenesis.