Brinker is a sequence-specific transcriptional repressor in the Drosophila embryo
Brinker is a sequence-specific transcriptional repressor in the Drosophila embryo
复制标题
DOI：
10.1101/gad.861201
复制
发表时间：
2001-02-01
影响因子：
10.5
通讯作者：
Ashe, HL
中科院分区：
文献类型：
--
作者：
Zhang, HL;Levine, M;Ashe, HL
DPP活性梯度规定了早期胚胎背侧外胚层基因表达的多个阈值。其中一些阈值依赖于一种可能的抑制因子Brinker，它在神经源性外胚层中表达，对母体背侧倾斜度和DPP信号做出反应。在这里，我们表明Brinker是一种序列特异的转录抑制因子。它与共有序列TGGCGc/TC/t结合，并通过保守的序列基序FKPY与Groucho辅阻遏子相互作用。一个最佳的Brinker结合位点包含在来自Tolloid基因的800个碱基的增强子中，该基因已被确定为Brinker阻遏基因的遗传靶标。在该位点含有点突变的Tolloid-LacZ转基因基因表现出一种扩大的表达模式，表明Brinker直接抑制Tolloid转录。我们讨论了转录抑制因子限制信号通路活动的其他例子。
A Dpp activity gradient specifies multiple thresholds of gene expression in the dorsal ectoderm of the early embryo. Some of these thresholds depend on a putative repressor, Brinker, which is expressed in the neurogenic ectoderm in response to the maternal Dorsal gradient and Dpp signaling. Here we show that Brinker is a sequence-specific transcriptional repressor. It binds the consensus sequence, TGGCGc/tc/t, and interacts with the Groucho corepressor through a conserved sequence motif, FKPY. An optimal Brinker binding site is contained within an 800-bp enhancer from the tolloid gene, which has been identified as a genetic target of the Brinker repressor. A tolloid-lacZ transgene containing point mutations in this site exhibits an expanded pattern of expression, suggesting that Brinker directly represses tolloid transcription. We discuss other examples of transcriptional repressors constraining the activities of signaling pathways.