Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium.
Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium.
复制标题
gp36（一种优先在血管内皮中表达的人粘蛋白型糖蛋白）的克隆和表征。
DOI：
10.1042/0264-6021:3410277
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
G. Herrler
中科院分区：
文献类型：
--
作者：
G. Zimmer;F. Oeffner;V. Messling;T. Tschernig;H. Gröne;H. Klenk;G. Herrler
粘蛋白型糖蛋白已在小鼠、大鼠和犬组织中被描述为分化抗原和流感病毒受体。我们从人胎盘RNA中克隆了一段编码相应人类蛋白的cDNA，这是一种含有162个氨基酸的i型完整膜蛋白。用cDNA克隆转染Madin-Darby犬肾细胞后，细胞表面表达了一个36 kDa的o糖基化唾液糖蛋白gp36，以及两个28和70 kDa的小亚型。gp36组织分布广泛，在肺、胎盘和骨骼肌中均有较强的表达，不同cDNA文库的PCR筛选结果表明。免疫组织化学检测人胎盘、肾、肺和鼻息肉的冷冻切片显示，gp36糖蛋白在血管内皮细胞的顶质膜上表达。gp36的表达并不局限于内皮细胞，肺泡上皮细胞也表达gp36。
A mucin-type glycoprotein has been described in murine, rat and canine tissues as a differentiation antigen and influenza-virus receptor. We have cloned a cDNA from human placenta RNA encoding the corresponding human protein, a type-I integral membrane protein of 162 amino acids. Madin-Darby canine kidney cells transfected with the cDNA clone directed the cell-surface expression of a 36-kDa O-glycosylated sialoglycoprotein, gp36, and two minor isoforms of 28 and 70 kDa. gp36 has a broad tissue distribution with strong expression in lung, placenta and skeletal muscle, as shown by PCR screening of different cDNA libraries. Immunohistochemical detection of gp36 in cryo-sections of human placenta, kidney, lung and nasal polyps showed that the glycoprotein is expressed at the apical plasma membrane of vascular endothelial cells. Expression of gp36 was not restricted to endothelial cells, as alveolar epithelial cells were found to express gp36 as well.