O2A PROGENITOR CELLS TRANSPLANTED INTO THE NEONATAL RAT-BRAIN DEVELOP INTO OLIGODENDROCYTES BUT NOT ASTROCYTES
O2A PROGENITOR CELLS TRANSPLANTED INTO THE NEONATAL RAT-BRAIN DEVELOP INTO OLIGODENDROCYTES BUT NOT ASTROCYTES
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DOI：
10.1073/pnas.90.1.50
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发表时间：
1993-01-01
影响因子：
11.1
通讯作者：
DEVELLIS, J
中科院分区：
文献类型：
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作者：
DELOSMONTEROS, AE;ZHANG, MS;DEVELLIS, J
双能 O2A 祖细胞分化为少突胶质细胞或 2 型星形胶质细胞已在中枢神经系统各个区域的细胞培养物中得到充分记录。一直缺乏证明其体内存在的适当工具。我们报告了一种体外-体内方法，该方法结合了用荧光染料固蓝对培养的 O2A 祖细胞富集群体进行稳定标记，然后将其移植到新生大鼠大脑中，这使我们能够研究大脑微环境对其谱系决定的影响。移植的细胞存活良好，移植后 21 天，几乎所有细胞的少突胶质细胞标记物半乳糖脑苷脂呈阳性。令人惊讶的是，快速蓝阳性移植细胞没有对星形胶质细胞标记物神经胶质原纤维酸性蛋白进行染色。这些结果表明O2A祖细胞的可塑性受到体内环境的限制，导致最初在体外描述的2型星形胶质细胞的发育排斥。
The differentiation of the bipotential O2A progenitor cell into an oligodendrocyte or a type 2 astrocyte has been well documented in cell cultures of various regions of the central nervous system. The appropriate tools to prove its existence in vivo have been lacking. We report on an in vitro-in vivo approach that combines stable labeling of an enriched population of cultured O2A progenitors by the fluorescent dye fast blue, followed by their transplantation into neonatal rat brains, which allowed us to study the influence of the brain microenvironment on their lineage decision. The grafted cells survived well and 21 days after grafting nearly all were positive for the oligodendroglial marker galactocerebroside. Surprisingly, the fast blue-positive grafted cells did not stain for the astroglial marker glial fibrillary acidic protein. These results indicate that the O2A progenitor's plasticity is restricted by the in vivo environment, resulting in the developmental exclusion of the type 2 astrocyte initially described in vitro.