Effects of dietary calcium and cadmium on cadmium accumulation, calcium and cadmium uptake from the water, and their interactions in juvenile rainbow trout
Effects of dietary calcium and cadmium on cadmium accumulation, calcium and cadmium uptake from the water, and their interactions in juvenile rainbow trout
复制标题
DOI：
10.1016/j.aquatox.2004.11.019
复制
发表时间：
2005-03-25
影响因子：
4.5
通讯作者：
Wood, CA
中科院分区：
文献类型：
--
作者：
Baldisserotto, B;Chowdhury, MJ;Wood, CA
本研究的目的是研究长期升高的饮食Ca2+（如CaCO3）单独和联合升高的饮食Cd对幼年虹鳟鱼（Oncorhynchus mykiss）的生存、生长和几个内部隔间中Cd和Ca2+积累的影响。此外，使用放射性示踪剂（Ca-45, Cd-65）监测急性水Cd暴露（50 μ g/L CdNO3，持续3小时）对新积累的水性Ca2+和Cd的短期分支吸收和内部分布的影响。分别饲喂20 mg Ca2+/g食物（对照）、50 mg Ca2+/g食物、300 μ g Cd/g食物和50 mg Ca2+/g + 300 μ g Cd/g食物，试验期30 d。对生长、死亡率或总体内Ca2+积累没有显著影响。在短期实验（3h）中，饮食中Ca2+、Cd或Ca2+ + Cd的升高都降低了水体内Ca2+的摄取，尽管抑制机制似乎有所不同。饲喂15天后效果显著，但在30天后减弱，除非饲料中Ca2+和Cd含量均升高。在急性Cd挑战期间，饲料中Ca2+含量升高对水中Cd的吸收只有适度影响，但极大地抑制了饲料中Cd的吸收和大多数内部组织的积累。没有一种处理饮食能阻止急性暴露于水体内Cd引起的水中Ca'吸收减少和内部组织中新的Ca2+积累。总之，水和饮食中Ca2+和Cd的影响之间存在复杂的相互作用。饮食中Ca2+的升高可以防止饮食和水体内Cd的吸收，而水体内和饮食中Cd的升高会导致水体内Ca2+的吸收减少。(c) 2004 Elsevier B.V.版权所有
The objective of this study was to examine the effects of chronically elevated dietary Ca2+ (as CaCO3) alone and in combination with elevated dietary Cd, on survival, growth, and Cd and Ca2+ accumulation in several internal compartments in juvenile rainbow trout (Oncorhynchus mykiss). In addition, effects on short-term branchial uptake and internal distribution of newly accumulated waterborne Ca2+ and Cd during acute waterborne Cd exposure (50 mu g/L as CdNO3 for 3 h) were monitored using radiotracers (Ca-45, Cd-65). Fish were fed with four diets: 20 mg Ca2+/g food (control), 50 mg Ca2+/g food, 300 mu g Cd/g food, and 50 mg Ca2+/g + 300 mu g Cd/g food for 30 days. There were no significant effects on growth, mortality, or total body Ca2+ accumulation. The presence of elevated Ca2+, Cd, or Ca2+ + Cd in the diet all reduced waterbome Ca2+ uptake in a short-term experiment (3h), though the inhibitory mechanisms appeared to differ. The effects were marked after 15 days of feeding, but attenuated by 30 days, except when the diet was elevated in both Ca2+ and Cd. The presence of elevated Ca2+ in the diet had only modest influence on Cd uptake from the water during acute Cd challenges but greatly depressed Cd uptake from the diet and accumulation in most internal tissues. None of the treatment diets prevented the decreases in waterborne Ca' uptake and new Ca2+ accumulation in internal tissues caused by acute exposure to waterbome Cd. In conclusion, there are complex interactions between waterborne and dietary effects of Ca2+ and Cd. Elevated dietary Ca2+ protects against both dietary and waterbome Cd uptake, whereas both waterbome and dietary Cd elevations cause reduced waterbome Ca2+ uptake. (c) 2004 Elsevier B.V. All rights reserved.