Preparation and characterization of dextran nanobubbles for oxygen delivery
Preparation and characterization of dextran nanobubbles for oxygen delivery
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DOI：
10.1016/j.ijpharm.2009.07.010
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发表时间：
2009-11-03
影响因子：
5.8
通讯作者：
Trotta, M.
中科院分区：
文献类型：
--
作者：
Cavalli, R.;Bisazza, A.;Trotta, M.
制备了具有葡聚糖壳和全氟戊烷核的葡聚糖纳米气泡，其中氧被储存。为了增加稳定性，还将聚乙烯吡咯烷酮作为稳定剂添加到制剂中。以罗丹明B为荧光标记物，制备荧光纳米泡。纳米气泡制剂显示出约500 nm的尺寸、负表面电荷和良好的载氧能力，对细胞系没有溶血活性或毒性作用。荧光标记的纳米气泡可以在Vero细胞中内化。在体外实验中，充氧纳米气泡能够在不同的低氧溶液中在不同的时间释放氧气。在2.5MHz的超声作用下，纳米气泡的氧释放动力学可以增强。充氧纳米气泡制剂可被提出用于各种疾病的治疗应用。(C)2009爱思唯尔有限公司版权所有。
Dextran nanobubbles were prepared with a dextran shell and a perfluoropentan core in which oxygen was stored. To increase the stability polyvinylpirrolidone was also added to the formulation as stabilizing agent. Rhodamine B was used as fluorescent marker to obtain fluorescent nanobubbles. The nanobubble formulations showed sizes of about 500 nm, a negative surface charge and a good capacity of loading oxygen, no hemolytic activity or toxic effect on cell lines. The fluorescent labelled nanobubbles could be internalized in Vero cells. Oxygen-filled nanobubbles were able to release oxygen in different hypoxic solutions at different time after their preparation in in vitro experiments. The oxygen release kinetics could be enhanced after nanobubble insonation with ultrasound at 2.5 MHz. The oxygen-filled nanobubble formulations might be proposed for therapeutic applications in various diseases. (C) 2009 Elsevier B.V. All rights reserved.