The NXSM recombinant inbred strains of mice: genetic profile for 58 loci including the Mtv proviral loci.
The NXSM recombinant inbred strains of mice: genetic profile for 58 loci including the Mtv proviral loci.
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NXSM 小鼠重组近交系：58 个基因座的遗传图谱，包括 Mtv 原病毒基因座。
DOI：
10.1093/genetics/125.2.431
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Lee,BK
中科院分区：
文献类型：
--
作者：
Eicher,EM;Lee,BK
我们报道了从NZB/BINRe和SM/J两个祖系衍生的17个重组近交系小鼠的构建，并对这个重组近交系品系进行了分型，命名为NXSM，分布在16个常染色体和X染色体上的58个座位上。构建了NZB/BINJ和SM/J两个回交组合，以确认NZB/BINJ和SM/J的染色体归属，并根据NXSM RI品系数据确定建议的基因顺序。根据这些结果，我们建议使用通过其他方法获得的数据来确认从RI菌株集分析中提出的染色体分配和基因顺序。我们还对乳腺肿瘤前病毒(MTV)基因座进行了NZB/BINJ和SM/J分型。这两个毒株都有三个先前描述的MTV基因座：MTV-7、MTV-14和MTV-17。此外，NZB/BINJ还含有先前描述的MTV-3和MTV-9基因座和两个新的MTV前病毒基因座：位于染色体(Chr)1上的MTV-27和位于X染色体上的MTV-28。SM/J包含前面描述的MTV-6和MTV-8基因座。发现并定位了4个形成水貂细胞病灶的小鼠白血病病毒基因座：Ltrm-1位于Chr 12，Ltrm-2位于Chr 16，Ltrm-3位于Chr 5，Ltrm-4位于Chr 13。Tgn位于Chr 15上Ly-6的近端。
We report the construction of 17 recombinant inbred (RI) strains of mice derived from the progenitor strains NZB/BINRe and SM/J and the typing of this RI strain set, designated NXSM, for 58 loci distributed on 16 autosomes and the X chromosome. Two backcrosses involving NZB/BINJ and SM/J were constructed to confirm chromosomal assignments and determine gene orders suggested from NXSM RI strain data. From these results we recommend that chromosomal assignments and gene orders suggested from analyses of RI strain sets be confirmed using data obtained by other means. We also typed NZB/BINJ and SM/J for mammary tumor proviral (Mtv) loci. Both strains share three previously described Mtv loci: Mtv-7, Mtv-14 and Mtv-17. In addition, NZB/BINJ contains the previously described Mtv-3 and Mtv-9 loci and two new Mtv proviral loci: Mtv-27 located on chromosome (Chr) 1 and Mtv-28 located on the X chromosome. SM/J contains the previously described loci Mtv-6 and Mtv-8. Four LTR, mink cell focus-forming murine leukemia viral loci were identified and mapped: Ltrm-1 on Chr 12, Ltrm-2 on Chr 16, Ltrm-3 on Chr 5, and Ltrm-4 on Chr 13. The Tgn locus was positioned proximal to the Ly-6 locus on Chr 15.