Monitoring the efficacy of ivermectin and albendazole against gastro intestinal nematodes of cattle in Northern Europe
Monitoring the efficacy of ivermectin and albendazole against gastro intestinal nematodes of cattle in Northern Europe
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DOI：
10.1016/j.vetpar.2008.10.030
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发表时间：
2009-03-09
影响因子：
2.6
通讯作者：
von Samson-Himmelstjerna, G.
中科院分区：
文献类型：
--
作者：
Demeler, J.;Van Zeveren, A. M. J.;von Samson-Himmelstjerna, G.
2006年和2007年，在德国、比利时和瑞典的牛场进行了伊维菌素（IVM）和苯并咪唑（BZ）粪便虫卵计数减少试验（FECRT），以调查胃肠道线虫对驱虫剂的耐药性。根据研究前足够数量的鸡蛋，每个国家选择3到10个农场。每个农场随机选择10-15只动物，皮下注射0.2 mg IVM/kg体重（Ivomec（R），Merial）。在第0天（给药）、第7天（比利时和瑞典）或第14天（德国）和第21天（德国、比利时和瑞典）从每只动物中单独采集粪便样品。在每次采样时进行粪便虫卵计数（FEC），以估计每克粪便中的虫卵数（EPG）和处理后的虫卵减少量。2006年使用IVM进行的FECRT显示，第7/14天虫卵计数平均减少69-100%（95%置信区间（CI）19-102），第21天平均减少35-96%（95% CI 0-102）。2007年，对存在驱虫剂耐药性问题的养殖场进行了复查，除一例外，2006年获得的所有结果均在2007年得到确认。从粪便培养物中获得的幼虫使用显微镜识别键或属特异性真实的时间PCR来识别。治疗后检测到的主要菌种是瘤孔古柏菌，但治疗后在德国3个农场和瑞典3个农场的样本中发现了奥斯特线虫。2007年，在德国和瑞典还进行了使用苯并咪唑的FECRT。在德国，口服Valbazen（R）（阿苯达唑，10%。Pfizer）以7.5mg阿苯达唑/kg体重的浓度使用;在Sweden Valbazen Vet（R）（阿苯达唑，10%，Orion Pharma）以8 mg/kg的剂量使用。对于苯并咪唑，在两个国家的所有受试农场中均获得了100%的疗效。这是在欧洲进行的首次多国牛驱虫药疗效研究报告。结果表明，由于目前药物的疗效可能不足，因此应更深入地进行驱虫疗效试验。(C)2008 Elsevier B. V.保留所有权利。
Faecal egg count reduction tests (FECRT) using ivermectin (IVM) and benzimidazole (BZ) were conducted to investigate the prevalence of anthelmintic resistance in gastrointestinal nematodes on cattle farms in Germany, Belgium and Sweden in 2006 and 2007. Based on sufficient numbers of eggs prior to the study, between 3 and 10 farms per country were selected. 10-15 animals were randomly selected per farm and subcutaneously treated with 0.2 mg IVM/kg bodyweight (Ivomec (R), Merial). Faecal samples were collected individually from every animal on day 0 (treatment), day 7 (Belgium & Sweden) or 14 (Germany), and day 21 (Germany, Belgium and Sweden). Faecal egg counts (FEC) were performed at each sampling occasion to estimate the eggs per gram of faeces (EPG) and the reduction of eggs after treatment. The FECRT using IVM in 2006 revealed mean reduction of egg counts between 69-100% on day 7/14 (95% confidence interval (CI) 19-102) and 35-96% (95% CI 0-102) on day 21. Farms with a suggested problem of anthelmintic resistance have been re-visited in 2007 and except for one case all results obtained in 2006 were confirmed in 2007. Larvae obtained from faecal cultures were identified using microscopic identification keys or genus-specific real time PCR. Cooperia oncophora was the predominant species detected after treatment, but Ostertagia ostertagi was found in samples on 3 farms in Germany and 3 farms in Sweden post-treatment.In 2007 additionally a FECRT using benzimidazoles was conducted in Germany and Sweden. In Germany oral Valbazen (R) (albendazole, 10%. Pfizer) was used at a concentration of 7.5 mg albendazole/kg bodyweight; in Sweden Valbazen Vet (R) (albendazole, 10%, Orion Pharma) at a dose of 8 mg/kg was used. For benzimidazoles an efficacy of 100% was obtained on all tested farms in both countries. This is the first report of a multinational anthelmintic efficacy investigation in cattle in Europe. The results suggest that testing of anthelmintic efficacy should be performed more intensively due to possible insufficient efficacy of current drugs. (C) 2008 Elsevier B.V. All rights reserved.