High-Fat Feeding Does Not Disrupt Daily Rhythms in Female Mice because of Protection by Ovarian Hormones.
High-Fat Feeding Does Not Disrupt Daily Rhythms in Female Mice because of Protection by Ovarian Hormones.
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DOI：
10.3389/fendo.2017.00044
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发表时间：
2017
影响因子：
5.2
通讯作者：
Pendergast JS
中科院分区：
文献类型：
--
作者：
Palmisano BT;Stafford JM;Pendergast JS
绝经后循环雌激素的丢失会增加女性的肥胖。轮班工作扰乱了昼夜节律，也增加了肥胖的风险。目前还不清楚卵巢激素是否与昼夜节律系统相互作用，以保护女性免受肥胖。在高脂肪喂养期间，雄性C57 BL/6 J小鼠发展为严重肥胖和日常节律紊乱。由于C57 BL/6 J雌性小鼠没有发展饮食诱导的肥胖（在8周的高脂肪喂养期间），我们首先确定雌性小鼠的每日节律是否抵抗高脂肪饮食的破坏。我们给雌性PERIOD 2：LUCIFERASE小鼠喂食45%高脂肪饮食1周，并测量每日节律。雌性小鼠在高脂肪喂养期间保持了与普通饲料喂养的雌性小鼠相似的进食行为和运动活动的稳健节律。此外，肝脏分子计时（PER 2：LUC）节律的阶段没有改变高脂肪喂养的女性。为了确定卵巢激素是否能保护雌性小鼠免受高脂喂养的日常节律，我们分析了卵巢切除小鼠的节律。在高脂喂养期间，卵巢切除雌性的进食行为和运动活动节律的幅度降低。在切除卵巢的雌性动物中，高脂喂养（而非食物）也使肝脏PER 2：LUC节律提前约4小时。这些数据共同表明，在高脂肪喂养期间，循环卵巢激素保护雌性小鼠日常节律的完整性。
Obesity in women is increased by the loss of circulating estrogen after menopause. Shift work, which disrupts circadian rhythms, also increases the risk for obesity. It is not known whether ovarian hormones interact with the circadian system to protect females from obesity. During high-fat feeding, male C57BL/6J mice develop profound obesity and disruption of daily rhythms. Since C57BL/6J female mice did not develop diet-induced obesity (during 8 weeks of high-fat feeding), we first determined if daily rhythms in female mice were resistant to disruption from high-fat diet. We fed female PERIOD2:LUCIFERASE mice 45% high-fat diet for 1 week and measured daily rhythms. Female mice retained robust rhythms of eating behavior and locomotor activity during high-fat feeding that were similar to chow-fed females. In addition, the phase of the liver molecular timekeeping (PER2:LUC) rhythm was not altered by high-fat feeding in females. To determine if ovarian hormones protected daily rhythms in female mice from high-fat feeding, we analyzed rhythms in ovariectomized mice. During high-fat feeding, the amplitudes of the eating behavior and locomotor activity rhythms were reduced in ovariectomized females. Liver PER2:LUC rhythms were also advanced by ~4 h by high-fat feeding, but not chow, in ovariectomized females. Together these data show circulating ovarian hormones protect the integrity of daily rhythms in female mice during high-fat feeding.