Mapping genes that control hormone-induced ovulation rate in mice.
Mapping genes that control hormone-induced ovulation rate in mice.
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DOI：
10.1095/biolreprod61.4.857
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发表时间：
1999-10
影响因子：
3.6
通讯作者：
J. Spearow;P. Nutson;W. Mailliard;M. Porter;M. Barkley
中科院分区：
文献类型：
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作者：
J. Spearow;P. Nutson;W. Mailliard;M. Porter;M. Barkley
本研究定位了控制C57 BL/6 J（B6）（HIOR = 54）和A/J品系小鼠（HIOR = 9）之间6倍遗传差异的数量性状基因座（QTL）。(The基因名称为排卵率诱导[ORI] QTL，基因符号为Oriq。）对167只（B6 xA）xA回交小鼠在165个位点进行了QTL连锁分析。控制卵丘中卵数的暗示性B6 ORI QTL定位如下：染色体（Chr）9（Oriq 1）上的Cyp 19和D9 Mit 4; Chr 2（Oriq 2）上的D2 Mit 433; Chr 6（Oriq 3）上的D 6 Mit 316; ChrX（Oriq 4）上的DXMit 22，并且分别与HIOR中的2.7、2.7、2.6和4.2个卵增加相关。基于复合区间作图，Oriq 3具有显著性（LOD = 3.45）。内源性促性腺激素成熟和eCG排卵的卵子数量的QTL连锁分析将Oriq 5定位到Chr 10，并暗示Oriq定位到Chr 6，7和X。这些数据为控制卵巢对促性腺激素反应性的主要差异的生殖QTL提供了第一个分子遗传标记。这些密切相关的同线分子标记将能够更准确地预测卵巢对促性腺激素的反应性，并为改善不同哺乳动物物种的生殖性能提供选择标准。
The present study mapped quantitative trait loci (QTL) that control 6-fold genetic differences in hormone-induced ovulation rate (HIOR) between C57BL/6J (B6) (HIOR = 54) and A/J strain mice (HIOR = 9). (The gene name is Ovulation Rate Induced [ORI] QTL and the gene symbol is Oriq.) QTL linkage analysis was conducted on 167 (B6xA)xA backcross mice at 165 loci. Suggestive B6 ORI QTL that control the number of eggs in cumulus mapped, as follows, near: Cyp19 and D9Mit4 on chromosome (Chr) 9 (Oriq1); D2Mit433 on Chr2 (Oriq2); D6Mit316 on Chr6 (Oriq3); DXMit22 on ChrX (Oriq4) and were associated with a 2.7, 2.7, 2.6, and 4.2 egg increases in HIOR, respectively. Oriq3 was significant (LOD = 3.45) based on composite interval mapping. QTL linkage analysis of the number of eggs matured by endogenous gonadotropins and ovulated by eCG mapped a significant Oriq5 to Chr 10 and suggestive Oriq to Chr 6, 7, and X. These data provide the first molecular genetic markers for reproductive QTL that control major differences in ovarian responsiveness to gonadotropins. These and closely linked syntenic molecular markers will enable a more accurate prediction of ovarian responsiveness to gonadotropins and provide selection criteria for improving reproductive performance in diverse mammalian species.