THE PACL GENE OF SYNECHOCOCCUS SP STRAIN PCC-7942 ENCODES A CA2+-TRANSPORTING ATPASE
THE PACL GENE OF SYNECHOCOCCUS SP STRAIN PCC-7942 ENCODES A CA2+-TRANSPORTING ATPASE
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DOI：
10.1128/jb.176.14.4430-4436.1994
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发表时间：
1994-07-01
影响因子：
3.2
通讯作者：
HOFFMAN, NE
中科院分区：
文献类型：
--
作者：
BERKELMAN, T;GARRETENGELE, P;HOFFMAN, NE
聚球藻的质膜囊泡具有依赖于ATP的钙摄取活性。PCC7942菌株。这种活性对崩解DB梯度和膜电位的试剂不敏感，但对钒酸盐敏感，表明该活性是由P型钙-ATPase催化的。从聚球藻中克隆了一个基因。通过使用基于在P型ATPase中保守的序列的简并寡核苷酸来分离PCC7942。该基因(PacL)编码的产物在结构上与真核细胞的钙-三磷酸腺苷酶相似。我们通过证明PACT被破坏的菌株没有与其质膜相关的Ca~(2+)-ATPase活性来证明PACL编码一个Ca~(2+)-ATPase。此外，通过将PACT导入聚球藻的第二个位点，恢复了Delta pacL菌株的Ca~(2+)-ATPase活性。PCC7942号染色体。
An ATP-dependent Ca2+ uptake activity was identified in plasma membrane vesicles prepared from Synechococcus sp. strain PCC 7942. This activity was insensitive to agents which collapse DB gradients and membrane potentials but sensitive to vanadate, indicating that the activity is catalyzed by a P-type Ca2+-ATPase. A gene was cloned from Synechococcus sp. strain PCC 7942 by using a degenerate oligonucleotide based on a sequence conserved among P-type ATPases. This gene (pacL) encodes a product similar in structure to eukaryotic Ca2+-ATPases. We have shown that pacl encodes a Ca2+-ATPase by demonstrating that a strain in which pact is disrupted has no Ca2+-ATPase activity associated with its plasma membrane. In addition, Ca2+-ATPase activity was restored to the Delta pacL strain by introducing pact into a second site in the Synechococcus sp. strain PCC 7942 chromosome.