DETERMINATION OF IGE ANTIBODIES TO THE BENZYL PENICILLOYL DETERMINANT - A COMPARISON BETWEEN POLY-L-LYSINE AND HUMAN SERUM-ALBUMIN AS CARRIERS
DETERMINATION OF IGE ANTIBODIES TO THE BENZYL PENICILLOYL DETERMINANT - A COMPARISON BETWEEN POLY-L-LYSINE AND HUMAN SERUM-ALBUMIN AS CARRIERS
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DOI：
10.1016/0022-1759(92)90311-g
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发表时间：
1992-08-30
影响因子：
2.2
通讯作者：
GARCIA, J
中科院分区：
文献类型：
--
作者：
BLANCA, M;MAYORGA, C;GARCIA, J
用固相法测定了两种不同载体聚-L-赖氨酸（PLL）和人血清白蛋白（HSA）对特异性IgE抗体与苄基青霉噻唑酰半抗原（BPO）结合的影响。使用的血清样本来自有青霉素即刻反应史且显示存在BPO IgE抗体的患者。以不同分子量的PLL和人血清白蛋白（HSA）为载体，制备了苄基青霉噻唑衍生纤维素片。用不同摩尔比的苄青霉素处理这些样品。在放射性过敏吸附试验（RAST）型测定中，使用血清池比较这些试剂对BPO特异性IgE的摄取。两个PLL系统和两个HSA系统最后使用26个个体血清进行比较。通过Kruskal-Wallis和Wilcoxon检验比较RAST值。通过测定Pearson相关系数评价4种不同检测方法之间的关系，并通过测定组内相关系数（ICC）评价一致性。通过Wilcoxon检验进行的平均值分析仅在比较不同载体测定时显示显著差异（P < 0.01）。所有检测方法之间的相关系数均具有显著性（P < 0.0001），但不同载体检测方法之间的ICC较低。这些结果表明，研究的载体（PLL和HSA）的性质影响RAST程序中结合IgE抗体的能力。观察到的差异表明，在测定BPO的IgE抗体时，一种缀合物不能替代另一种缀合物，BPO-PLL是优选的。
The influence of two different carriers, poly-L-lysine (PLL) and human serum albumin (HSA) in the binding of specific IgE antibodies to the benzyl penicilloyl hapten (BPO) was determined in a solid-phase assay. Serum samples from patients with a history of immediate reaction to penicillin and which had shown the presence of IgE antibodies to BPO were used. Benzyl penicilloyl derivatized cellulose discs were prepared using PLL of different molecular weight and HSA as carriers. These were treated with different molar ratios of benzyl penicillin. These reagents were compared for uptake of BPO-specific IgE using a pool of sera in a radioallergosorbent test (RAST) type assay. Two PLL systems and two HSA systems were finally compared using 26 individual sera. RAST values were compared by Kruskal-Wallis and Wilcoxon tests. The relationships between the four different assays were evaluated by determining Pearson correlation coefficients and the concordance by determining intraclass correlation coefficients (ICC). Analysis of means by the Wilcoxon test revealed significant differences (P < 0.01) only when the different carrier assays were compared. The correlation coefficients between all the assays were significant (P < 0.0001), but the ICC was low when the different carrier assays were compared. These results indicate that the nature of the carriers studied (PLL and HSA) influences the capacity for binding IgE antibodies in the RAST procedure. The differences observed indicate that one conjugate cannot be substituted for the other in the determination of IgE antibodies to BPO and that BPO-PLL is preferable.