Oocytes of baboon fetal primordial ovarian follicles express estrogen receptor β mRNA
Oocytes of baboon fetal primordial ovarian follicles express estrogen receptor β mRNA
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DOI：
10.1007/s12020-008-9081-y
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发表时间：
2008-06-01
期刊：
影响因子：
3.7
通讯作者：
Pepe, Gerald J.
中科院分区：
文献类型：
--
作者：
Bocca, Silvina M.;Billiar, Reinhart B.;Pepe, Gerald J.
在雌激素抑制狒狒的胎儿卵巢中，我们先前已经表明卵泡数量比雌激素充足的动物低50%，并且含有微绒毛数量减少的卵母细胞。在狒狒胎儿卵巢中，虽然通过免疫细胞化学在颗粒细胞中检测到雌激素受体（ER）α和β，但不知道卵母细胞是否表达ER。由于雌激素的作用是通过与细胞特异性受体的相互作用介导的，因此本研究确定了在妊娠第165天（足月=第184天）获得的狒狒胎儿卵巢的卵母细胞中是否表达ER α/β mRNA。通过激光捕获显微切割分离原始卵泡的卵母细胞核和细胞质，通过RT-PCR测定ER α、ER β、加塔-4（颗粒细胞特异性标记物）mRNA和18 S rRNA，并通过测序验证产物。5/5胎卵母细胞ER β mRNA表达。相反，胎儿卵母细胞不表达ER α mRNA。尽管18 S rRNA在所有卵母细胞中表达，但在卵母细胞中未检测到加塔-4 mRNA，仅在颗粒细胞中检测到，证实了采样的卵母细胞的纯度。我们得出结论，卵母细胞的胎儿狒狒卵巢表达ER β mRNA，从而提供了一种机制，雌激素调节卵母细胞的功能，例如微绒毛发育。
In fetal ovaries of estrogen-suppressed baboons, we have previously shown that follicle numbers were 50% lower than in estrogen-replete animals and contained oocytes with a reduced number of microvilli. In the baboon fetal ovary, although estrogen receptor (ER)alpha and beta have been detected by immunocytochemistry in granulosa cells, it is not known whether oocytes express ER. Because the actions of estrogen are mediated by interaction with cell-specific receptors, the current study determined whether ER alpha/beta mRNA were expressed in oocytes of baboon fetal ovaries obtained on day 165 (term = day 184) of gestation. Oocyte nuclei and cytoplasm from primordial follicles were isolated by laser capture microdissection and ER alpha, ER beta, GATA-4 (granulosa cell specific marker) mRNAs, and 18S rRNA determined by RT-PCR and products verified by sequencing. ER beta mRNA was expressed in oocytes of 5 of 5 fetuses. In contrast, fetal oocytes did not express ER alpha mRNA. Although 18S rRNA was expressed in all oocytes, GATA-4 mRNA was not detected in oocytes and only detected in granulosa cells confirming purity of oocytes sampled. We conclude that oocytes of the fetal baboon ovary express ER beta mRNA, thereby providing a mechanism by which estrogen regulates oocyte function, e.g. microvillus development.