Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1
Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1
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DOI：
10.1016/j.jbiosc.2009.05.003
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发表时间：
2009-11-01
影响因子：
2.8
通讯作者：
Sonomoto, Kenji
中科院分区：
文献类型：
--
作者：
Oshiro, Mugihito;Shinto, Hideaki;Sonomoto, Kenji
利用WinBEST-KIT软件建立了乳酸乳球菌IO-1木糖代谢动力学模拟模型。该模型是通过将在初始木糖浓度为20.3-57.8 g/l的培养基中生长的分批培养物中代谢物的实验时程数据与相应的计算数据进行比较而开发的。通过引入底物活化、底物抑制和产物抑制项，修正后的模型显示代谢物的实验时程与计算数据之间的平方相关系数（r（2））为0.929。因此，修改后的模型被认为是最好的候选人之一，动力学模拟描述的代谢物的动态行为。敏感性分析表明，丙酮酸流量分布是重要的较高的乳酸生产。为了证实我们的动力学模型的有效性，灵敏度分析的结果进行了比较，在增加乳酸生产过程中观察到的酶活性，通过添加天然橡胶血清粉的木糖培养基。丙酮酸通量分布的实验结果与灵敏度分析的预测一致。(C)2009年，生物技术学会，日本。All rights reserved.
We proposed a kinetic simulation model of xylose metabolism in Lactococcus lactis IO-1 that describes the dynamic behavior of metabolites using the simulator WinBEST-KIT. This model was developed by comparing the experimental time-course data of metabolites in batch cultures grown in media with initial xylose concentrations of 20.3-57.8 g/l with corresponding calculated data. By introducing the terms of substrate activation, substrate inhibition, and product inhibition, the revised model showed a squared correlation coefficient (r(2)) of 0.929 between the experimental time-course of metabolites and the calculated data. Thus, the revised model is assumed to be one of the best candidates for kinetic simulation describing the dynamic behavior of metabolites. Sensitivity analysis revealed that pyruvate flux distribution is important for higher lactate production. To confirm the validity of our kinetic model, the results of the sensitivity analysis were compared with enzyme activities observed during increasing lactate production by adding natural rubber serum powder to the xylose medium. The experimental results on pyruvate flux distribution were consistent with the prediction by sensitivity analysis. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.