The effects of moderate exercise on chronic stress-induced intestinal barrier dysfunction and antimicrobial defense
The effects of moderate exercise on chronic stress-induced intestinal barrier dysfunction and antimicrobial defense
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DOI：
10.1016/j.bbi.2013.11.013
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发表时间：
2014-07-01
影响因子：
15.1
通讯作者：
Chen, Peijie
中科院分区：
文献类型：
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作者：
Luo, Beibei;Xiang, Dao;Chen, Peijie
本研究的目的是观察适度运动对重复束缚应激(RRS)诱导的肠屏障功能障碍的影响，并探讨可能的机制。将6周龄雄性Balb/c小鼠随机分为7组：CON组作为对照组，不干预；RRS组每天游泳6h，连续7d；RRS+SWIFE组在RRS前每天游泳30min；CON+SWIFE组每天游泳30min；其余组在恢复1、3、6h处死前进行1次30min游泳。用FITC-葡聚糖定量测定肠道通透性。培养的肠系膜淋巴结(MLN)细菌集落形成单位(CFU)定量和荧光原位杂交(FISH)法检测细菌移位。采用实时定量聚合酶链式反应(Q-PCR)检测30 mm游泳前和训练后1h小肠组织中抗菌相关基因的表达。用酶联免疫吸附试验(ELISA)检测游泳前、游泳后1h、3h和6h 5个差异有统计学意义的基因的蛋白表达。在RRS减少细菌移位和维持肠道通透性之前，每天游泳30分钟。游泳30min后，4种抗菌肽(α-防御素5、β-防御素1、RegIIIβ和RegIII Gamma)的基因表达和蛋白水平均显著升高。总而言之，适度运动可减轻慢性应激所致的小鼠肠道屏障功能障碍，这可能是由于增强了小肠的抗微生物反应。(C)2013 Elsevier Inc.保留所有权利。
The purpose of this study was to examine the effect of moderate exercise on repeated restraint stress (RRS)-induced intestinal barrier dysfunction and explore possible mechanisms in a mouse model. Male Balb/c mice (6 weeks) were randomized into 7 groups: CON functioned as controls with no intervention; RRS was subjected to 6 h per day RRS for 7 consecutive days; RRS+SWIM received 30 min per day of swimming prior to RRS; CON+SWIM only received 30 min per day of swimming; and the other groups received one session of 30 min swimming prior to sacrifice at 1-, 3- and 6 h recovery. Intestinal permeability was quantified with FITC-dextran. Bacterial translocation was determined by quantification of bacterial colony forming units (CFUs) in cultured mesenteric lymph nodes (MLN), and with fluorescence in situ hybridization (FISH). Antimicrobial related gene expression at baseline and 1 h after one session of 30 mm swimming was tested by quantitative real-time polymerase chain reaction (Q-PCR) in small intestinal segments. Protein expression of 5 genes with statistically significant increase was measured at baseline, and 1-, 3- and 6 h post-swimming using enzyme-linked immunosorbent assay (ELISA). Thirty minutes per day of swimming before RRS attenuated bacterial translocations and maintained intestinal permeability. Gene expression and protein levels for four antimicrobial peptides (alpha-defensin 5, beta-defensin 1, RegIII beta and RegIII gamma) were significantly increased after one 30 min swimming session. In conclusion, moderate exercise attenuated chronic stress-induced intestinal barrier dysfunction in mice, possibly due to augmentation of antimicrobial responses in the small intestine. (C) 2013 Elsevier Inc. All rights reserved.