Host-pathogen reorganisation during host cell entry by Chlamydia trachomatis.
Host-pathogen reorganisation during host cell entry by Chlamydia trachomatis.
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DOI：
10.1016/j.micinf.2015.08.004
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发表时间：
2015-11
影响因子：
5.8
通讯作者：
Hayward RD
中科院分区：
文献类型：
--
作者：
Nans A;Ford C;Hayward RD
沙眼衣原体是一种专性细胞内细菌病原体，在世界范围内仍然是一个重大的公共卫生负担。感染早期的一个关键事件是衣原体进入非吞噬性宿主上皮细胞。与其他革兰氏阴性菌一样，沙眼衣原体使用III型分泌系统（T3SS）将毒力效应蛋白输送到宿主细胞中。这些效应物触发细菌摄取并促进细菌在宿主细胞内的生存和复制。在这篇综述中，我们重点介绍了最近的低温电子断层扫描技术，该技术为衣原体与其宿主之间的初始相互作用提供了惊人的见解。我们描述了细胞外沙眼衣原体（EBs）的极化结构，以及EB表面T3SS复合物的超分子组织，以及伴随细菌内化和EB被封装到早期细胞内液泡的宿主和病原体结构的变化。最后，我们考虑了进一步了解沙眼衣原体进入机制的意义，以及这可能与其他细菌和病毒的机制有关。
Chlamydia trachomatis is obligate intracellular bacterial pathogen that remains a significant public health burden worldwide. A critical early event during infection is chlamydial entry into non-phagocytic host epithelial cells. Like other Gram-negative bacteria, C. trachomatis uses a type III secretion system (T3SS) to deliver virulence effector proteins into host cells. These effectors trigger bacterial uptake and promote bacterial survival and replication within the host cell. In this review, we highlight recent cryo-electron tomography that has provided striking insights into the initial interactions between Chlamydia and its host. We describe the polarised structure of extracellular C. trachomatis elementary bodies (EBs), and the supramolecular organisation of T3SS complexes on the EB surface, in addition to the changes in host and pathogen architecture that accompany bacterial internalisation and EB encapsulation into early intracellular vacuoles. Finally, we consider the implications for further understanding the mechanism of C. trachomatis entry and how this might relate to those of other bacteria and viruses.