Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits
Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits
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DOI：
10.1038/jcbfm.2008.167
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发表时间：
2009-04-01
影响因子：
6.3
通讯作者：
Abe, Koji
中科院分区：
文献类型：
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作者：
Sakurai, Masahiro;Kawamura, Takae;Abe, Koji
脊髓损伤的机制被认为与脊髓运动神经元细胞对缺血的脆弱性有关。然而，这种脆弱性的机制尚未完全了解。我们通过免疫组织化学分析DJ-1、PINK 1和α-突触核蛋白研究了神经元死亡的可能机制。我们使用了15分钟的兔脊髓缺血模型，使用球囊导管。进行了DJ-1、PINK 1和α-突触核蛋白的蛋白质印迹分析; DJ-1、PINK 1和α-突触核蛋白免疫反应性的时间曲线;以及双标记荧光免疫细胞化学研究。蛋白质印迹分析显示，假手术脊髓中DJ-1、PINK 1和α-突触核蛋白的免疫反应性很少。然而，它们在短暂缺血后8h变得明显，在1天恢复到基线水平。双标记荧光免疫细胞化学研究显示，再灌注8 h，同一运动神经元中DJ-1和PINK 1，以及DJ-1和α-Synuclein均呈阳性表达，并最终死亡。再灌注早期运动神经元中DJ-1和PINK 1蛋白的诱导可能反映了氧化应激，而α-Synuclein蛋白的诱导可能与短暂性脊髓缺血后程序性细胞死亡的变化有关。
The mechanism of spinal cord injury has been thought to be related to the vulnerability of spinal motor neuron cells against ischemia. However, the mechanisms of such vulnerability are not fully understood. We investigated a possible mechanism of neuronal death by immunohistochemical analysis for DJ-1, PINK1, and alpha-Synuclein. We used a 15-min rabbit spinal cord ischemia model, with use of a balloon catheter. Western blot analysis for DJ-1, PINK1, and alpha-Synuclein; temporal profiles of DJ-1, PINK1, and alpha-Synuclein immunoreactivity; and double-label fluorescence immunocytochemical studies were performed. Western blot analysis revealed scarce immunoreactivity for DJ-1, PINK1, and alpha-Synuclein in the sham-operated spinal cords. However, they became apparent at 8 h after transient ischemia, which returned to the baseline level at 1 day. Double-label fluorescence immunocytochemical study revealed that both DJ-1 and PINK1, and DJ-1 and alpha-Synuclein were positive at 8 h of reperfusion in the same motor neurons, which eventually die. The induction of DJ-1 and PINK1 proteins in the motor neurons at the early stage of reperfusion may indicate oxidative stress, and the induction of alpha-Synuclein may be implicated in the programmed cell death change after transient spinal cord ischemia.