Apoptosis plays an important role in experimental rabies virus infection
Apoptosis plays an important role in experimental rabies virus infection
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DOI：
10.1128/jvi.71.7.5603-5607.1997
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发表时间：
1997-07-01
影响因子：
5.4
通讯作者：
Rossiter, JP
中科院分区：
文献类型：
--
作者：
Jackson, AC;Rossiter, JP
培养的大鼠前列腺腺癌（AT 3）细胞感染的挑战病毒标准（CVS）株的固定狂犬病病毒表现出特征性的形态特征的细胞凋亡，寡核小体DNA片段化的证据，和Bax蛋白的表达。CVS感染的Bcl-2转染的AT 3细胞没有表现出这些功能。脑内接种CVS的成年ICR小鼠表现出细胞凋亡、DNA片段化和神经元Bax表达增加的形态学变化，其中海马和大脑皮质的变化最为显著。超微结构上，一些神经元表现出更典型的坏死形态特征。这些研究为细胞凋亡在狂犬病病毒感染的发病机制中发挥重要作用提供了证据。
Cultured rat prostatic adenocarcinoma (AT3) cells infected with the challenge virus standard (CVS) strain of fixed rabies virus showed characteristic morphologic features of apoptosis, evidence of oligonucleosomal DNA fragmentation, and expression of the Bax protein. CVS-infected Bcl-2-transfected AT3 cells did not demonstrate these features. Adult ICR mice inoculated intracerebrally with CVS showed morphologic changes of apoptosis, DNA fragmentation, and increased Bax expression in neurons, with changes most marked in the hippocampus and cerebral cortex. Ultrastructurally, some neurons demonstrated morphologic features more typical of necrosis. These studies provide evidence that apoptosis plays an important role in the pathogenesis of rabies virus infection.