HEALING OF RAT SKIN WOUNDS
HEALING OF RAT SKIN WOUNDS
复制标题
DOI：
10.1097/00000658-196502000-00019
复制
发表时间：
1965-01-01
影响因子：
9
通讯作者：
ROSEN, H
中科院分区：
文献类型：
--
作者：
LEVENSON, SM;GEEVER, EF;ROSEN, H
大鼠实验性皮肤伤口显示胶原纤维的数量、口径和密度的渐进性显微变化，长达1年。变化率各不相同;直到42天，其速度比之后快得多，但在伤口中细胞活性和血管分布消退到邻近未受累真皮的水平之后仍然可以检测到。从术后第14天到第42天，伤口的断裂强度迅速增加，几乎呈线性;在接下来的4周内，这种增加率下降，伤口在3个月内几乎达到最大强度。整个曲线是S形的。我们得出结论，伤口胶原蛋白增加6至7周后的伤口。在愈合期间，拉伸强度增益可能与断裂强度增益平行，但伤口厚度测量的不确定性使得精确的拉伸强度曲线不如断裂强度曲线精确。福尔马林固定可增加伤口的断裂强度，但对早期伤口（长达6或7周）的影响比晚期伤口大得多。这正好对应于伤口胶原蛋白增加的时期，表明未成熟和次最大交联的胶原蛋白受福尔马林（本身是交联剂）的影响最大。不同年龄动物的未受伤皮肤的断裂强度几乎与动物的体重呈线性关系;它与皮肤厚度、水分浓度、重量和皮条中的羟脯氨酸浓度相关性较差;最佳相关性是与皮条中的羟脯氨酸含量。这与胶原蛋白主要负责结缔组织强度的观点一致。
Experimental dermal wounds in rats showed progressive microscopic changes in number, caliber and density of the collagen fibers up to 1 year. The rate of change varied; it was much more rapid up to 42 days than afterward, but still detectable after that time when cellular activity and vascularity in the wound had subsided to that of the adjacent uninvolved derma. The breaking strength of wounds increases rapidly and almost linearly, from about the 14th to the 42nd postoperative day; this rate of increase then declines during the next 4 weeks, and the wound attains almost maximal strength in 3 months. The entire curve is sigmoid. We conclude that wound collagen increases for 6 to 7 weeks after the wound. Tensile strength gain probably parallels the breaking strength gain over the healing period, but uncertainties in the wound thickness measurements make the exact tensile strength curve less precise than the breaking strength curve. Formalin fixation increases wound breaking strength, but has a proportionately much greater effect on early wounds (up to 6 or 7 weeks) than later ones. This corresponds exactly to the period of increasing wound collagen, suggesting that immature and submaximally crosslinked collagen is most affected by formalin, itself a crosslinking agent. The breaking strength of unwounded skin from animals of different ages varies almost linearly with the weight of the animals; it correlates poorly with skin thickness, water concentration, weight and hydroxyproline concentration in the skin strip; the best correlation was with the hydroxyproline content of the strip. This is consistent with the view that collagen is primarily responsible for the strength of connective tissue.