CELLULOSE ACETATE-COATED FUSED-SILICA CAPILLARIES FOR THE SEPARATION OF PROTEINS BY CAPILLARY ZONE ELECTROPHORESIS
CELLULOSE ACETATE-COATED FUSED-SILICA CAPILLARIES FOR THE SEPARATION OF PROTEINS BY CAPILLARY ZONE ELECTROPHORESIS
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DOI：
10.1016/0021-9673(94)01185-h
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发表时间：
1995-03-31
影响因子：
4.1
通讯作者：
POPPE, H
中科院分区：
文献类型：
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作者：
BUSCH, MHA;KRAAK, JC;POPPE, H
在熔融石英毛细管表面涂上醋酸纤维素薄膜，进行了毛细管区带电泳分离蛋白质的试验。涂层程序非常简单，仅涉及用醋酸纤维素溶液填充毛细管，然后用氦气冲洗毛细管。涂层似乎有效地掩蔽了基础硅烷醇基团对碱性蛋白质的作用;核糖核酸酶A的柱效率达到每米10（6）个板。涂层的高效率、批次间和批次间再现性以及长期稳定性是该方法的有利特征。该涂覆程序提供了一种简单、稳定且易于重现的表面失活方法，并且可以与其他纤维素衍生物如三乙酸纤维素或交联羟丙基纤维素一起应用。该膜也可用于屏蔽中空聚丙烯纤维的表面。不幸的是，这种皮肤涂层在pH 7.5以上被破坏，因此不能推荐用于碱性pH下的区带电泳或等电聚焦。
Thin-film coatings of cellulose acetate on the surface of fused-silica capillaries were tested for the separation of proteins by capillary zone electrophoresis. The coating procedure is very simple and only involves the filling of the capillary with cellulose acetate solution, followed by flushing the capillary with helium. The coating appears to mask the underlying silanol groups towards basic proteins effectively; column efficiencies up to 10(6) plates per metre were achieved for ribonuclease A. The high efficiency, the batch-to-batch and the run-to-run reproducibility and the long-term stability of the coating are advantageous features of the method. The coating procedure provides a simple, stable and easy to reproduce method of surface deactivation and can be applied with other cellulose derivatives such as cellulose triacetate or cross-linked hydroxypropylcellulose. The films can also be applied to shield the surface of hollow polypropylene fibres. Unfortunately, this skin coating is destroyed above pH 7.5 and therefore cannot be recommended for zone electrophoresis at alkaline pH or for isoelectric focusing.