A novel member of the family Hepeviridae from cutthroat trout (Oncorhynchus clarkii)
A novel member of the family Hepeviridae from cutthroat trout (Oncorhynchus clarkii)
复制标题
DOI：
10.1016/j.virusres.2011.03.019
复制
发表时间：
2011-06-01
期刊：
影响因子：
5
通讯作者：
Winton, James
中科院分区：
文献类型：
--
作者：
Batts, William;Yun, Susan;Winton, James
从1988年开始，在美国加州州，奇努克鲑鱼胚胎（CHSE-214）细胞系被用于从产卵的成年鳟鱼中分离一种新病毒。这种小而圆的病毒被称为克氏虹鳟病毒（Oncorhynchus clarkii）（DV），与疾病无关，但随后发现在美国西部越来越多的鳟鱼种群中存在，这可能是由于改进的监测活动和将受感染的卵运往新的地点。本文报道了1988年Heenan Lake分离株DV的全长基因组由7269个核苷酸的正链单链RNA组成，其起始于5'非翻译区（UTR），随后是三个开放阅读框（ORF），一个3'非翻译区和一个polyA尾。DV的基因组在大小和组织上与戊型肝炎病毒（HEV）相似，其具有最高的核苷酸和氨基酸序列同一性。与人类、啮齿动物或禽类肝炎病毒的基因组相似，ORF 1编码一个大的非结构多聚蛋白，包括保守的甲基转移酶、蛋白酶、解旋酶和聚合酶结构域，而ORF 2编码结构衣壳蛋白，ORF 3编码磷蛋白。总之，我们的数据表明DV显然是肝炎病毒科的成员，尽管与哺乳动物或禽类肝炎病毒的ORF的氨基酸序列同一性水平（13-27%）可能足够低以保证创建新属。我们还进行了系统发育分析，使用ORF 1内的262个核苷酸的区域，从7个品种的鳟鱼饲养在美国西部的不同地理位置的63株DV。虽然序列分为两个遗传分支，但总体核苷酸多样性较低（低于8.4%），许多分离株仅相差1-2个核苷酸，表明流行病学联系。最后，我们表明DV能够形成CHSE-214细胞系的持续感染培养物，该细胞系可用于研究人类或其他动物的肝炎病毒感染的生物学或治疗。由爱思唯尔公司出版
Beginning in 1988, the Chinook salmon embryo (CHSE-214) cell line was used to isolate a novel virus from spawning adult trout in the state of California, USA. Termed the cutthroat trout (Oncorhynchus clarkii) virus (DV), the small, round virus was not associated with disease, but was subsequently found to be present in an increasing number of trout populations in the western USA, likely by a combination of improved surveillance activities and the shipment of infected eggs to new locations. Here, we report that the full length genome of the 1988 Heenan Lake isolate of DV consisted of 7269 nucleotides of positive-sense, single-stranded RNA beginning with a 5' untranslated region (UTR), followed by three open reading frames (ORFs), a 3' UTR and ending in a polyA tail. The genome of DV was similar in size and organization to that of Hepatitis E virus (HEV) with which it shared the highest nucleotide and amino acid sequence identities. Similar to the genomes of human, rodent or avian hepeviruses, ORF 1 encoded a large, non-structural polyprotein that included conserved methyltransferase, protease, helicase and polymerase domains, while ORF 2 encoded the structural capsid protein and ORF 3 the phosphoprotein. Together, our data indicated that DV was clearly a member of the family Hepeviridae, although the level of amino acid sequence identity with the ORFs of mammalian or avian hepeviruses (13-27%) may be sufficiently low to warrant the creation of a novel genus. We also performed a phylogenetic analysis using a 262 nt region within ORF 1 for 63 isolates of DV obtained from seven species of trout reared in various geographic locations in the western USA. While the sequences fell into two genetic clades, the overall nucleotide diversity was low (less than 8.4%) and many isolates differed by only 1-2 nucleotides, suggesting an epidemiological link. Finally, we showed that DV was able to form persistently infected cultures of the CHSE-214 cell line that may have use in research on the biology or treatment of hepevirus infections of humans or other animals. Published by Elsevier B.V.