Chromosomal distributions of oligo-Am1 and (TTG)6 trinucleotide and their utilization in genome association analysis of sixteen Avena species
Chromosomal distributions of oligo-Am1 and (TTG)6 trinucleotide and their utilization in genome association analysis of sixteen Avena species
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寡聚Am1和(TTG)(6)三核苷酸的染色体分布及其在十六种燕麦属基因组关联分析中的应用
DOI：
10.1007/s10722-018-0639-0
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发表时间：
2018-08-01
影响因子：
2
通讯作者：
Chen, Liang
中科院分区：
文献类型：
--
作者：
Luo, Xiaomei;Tinker, Nick A.;Chen, Liang
采用荧光原位杂交技术研究了燕麦属七个二倍体物种（AA 或 CC 基因组）、七个四倍体物种（AABB 或 AACC 基因组）和两个六倍体物种（AACCDD 基因组）中期染色体中寡聚 Am1 和 (TTG)(6) 三核苷酸重复的物理位置。寡聚 Am1 探针产生的信号在几乎整个 C 基因组染色体上特别富集，而 (TTG)(6) 探针位于着丝粒周围 (M)，偶尔也位于端粒 (T) 染色体区域，但与 C 基因组的匹配度较低。所有物种在M区都具有(TTG)(6)位点，CC、AABB和AACCDD物种在T区也具有(TTG)(6)位点。 (TTG)(6) 信号数在 AA 和 CC 物种中是恒定的，但信号强度略有不同，而 (TTG)(6) 信号模式在 AABB、AACC 和 AACCDD 物种中显示出广泛的多样性。探针杂交结果提供了可用于将基因组序列物理分配到染色体的关键信息。
Fluorescence in situ hybridization was used to investigate the physical location of oligo-Am1 and (TTG)(6) trinucleotide repeats in the metaphase chromosomes of seven diploid species (AA or CC genomes), seven tetraploid species (AABB or AACC genomes), and two hexaploid species (AACCDD genomes) belonging to the genus Avena. The oligo-Am1 probe produced signals that were particularly enriched on almost whole C-genome chromosomes, whereas the (TTG)(6) probe was located in the pericentromeric (M), and, occasionally, their telomeric (T) chromosome regions, but showed low matching to C genome. All the species possessed (TTG)(6) loci in M regions, and the CC, AABB, and AACCDD species also possessed (TTG)(6) loci in T regions. The (TTG)(6) signal number is constant in both the AA and CC species but slightly differs in signal intensity, whereas the (TTG)(6) signal pattern shows wide diversity in the AABB, AACC and AACCDD species. The probe hybridization results provide key information that can be used in the physical assignment of genome sequences to chromosomes.