CHARACTERIZATION OF INTERSTITIAL COLLAGENASES IN JAW CYST WALL
CHARACTERIZATION OF INTERSTITIAL COLLAGENASES IN JAW CYST WALL
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DOI：
10.1111/j.1600-0722.1995.tb00015.x
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发表时间：
1995-06-01
影响因子：
1.9
通讯作者：
SORSA, T
中科院分区：
文献类型：
--
作者：
TERONEN, O;SALO, T;SORSA, T
制备了 14 个颌骨囊肿标本的中性盐提取物。组织病理学分析显示，标本包括6个根性囊肿、6个含齿囊肿、1个残留囊肿和1个牙源性角化囊肿。对 1 个根尖肉芽肿、1 个牙囊和临床健康口腔粘膜样本进行类似处理并用作对照。通过 SDS-PAGE 监测溶液中 I 型和 II 型胶原蛋白特异性降解产物的形成来测量胶原酶活性，结果表明所有囊肿提取物都含有胶原酶，其中一些是内源激活的。囊壁胶原酶优先降解 I 型胶原而不是 II 型胶原，这表明降解是由于 MMP-1（基质金属蛋白酶-1）而不是 MMP-8 类型造成的。囊肿胶原酶的多西环素抑制特性进一步支持了这一点，该特性与 MMP-1 相似。部分囊壁胶原酶呈潜在酶原形式，可能至少部分源自新合成的细胞内胶原酶库。潜伏的囊肿胶原酶可被氯化苯汞有效激活，硫代葡萄糖金 (I) 和 NaOCl 也可在较小程度上激活潜伏的囊肿胶原酶。使用来自人多形核中性粒细胞 (MMP-8) 和成纤维细胞 (MMP-1) 的胶原酶特异性抗体进行蛋白质印迹，显示出典型的 55/45 kDa 双联体；还发现了潜在 80 kDa 形式的 MMP-8 和片段化为 65 kDa 的活性物质。这些结果表明囊肿壁中存在 MMP-1 和较小程度的 MMP-8 型胶原酶。囊肿壁中部分活化的间质胶原酶的存在可能有助于囊肿壁重塑，因此可能部分调节囊肿生长。
Neutral salt extracts of 14 specimens of jaw cysts were prepared. Histopathological analysis showed that the specimens consisted of 6 radicular cysts, 6 dentigerous cysts, 1 residual cyst, and 1 odontogenic keratocyst. One periapical granuloma, 1 dental follicle and a sample of clinically healthy oral mucosa were similarly processed and used as controls. Measurement of collagenase activity by monitoring the formation of specific degradation products of type I and II collagen in solution by SDS-PAGE demonstrated that all the cyst extracts contained collagenase, some of which was endogenously activated. Cyst wall collagenase preferably degraded type I over type II collagen, which suggests that the degradation was due to MMP-1 (matrix metalloproteinase-1) rather than the MMP-8 type. This was further supported by the doxycycline-inhibition profile of cyst collagenase, which was similar to that of MMP-1. Part of the cyst wall collagenase was in latent proenzyme form and probably derived, at least in part, from the newly synthesized intracellular collagenase pool. Latent cyst collagenase was efficiently activated with phenylmercuric chloride and to a lesser extent by gold (I) thioglucose and NaOCl. Western-blotting, using specific antibodies against collagenase from human polymorphonuclear neutrophilic leukocytes (MMP-8) and from fibroblasts (MMP-1), revealed a typical 55/45 kDa doublet; also MMP-8 in the latent 80 kDa form and fragmented to 65 kDa active species were found. These results suggest the presence of MMP-1 and, to a lesser extent, MMP-8 type collagenase in the cyst wall. The presence of partially activated interstitial collagenases in the cyst wall may contribute to cyst wall remodeling and may thus, in part, regulate cyst growth.