Variations in parametrial white adipose tissue mass during the mouse estrous cycle:: relationship with the expression of peroxisome proliferator-activated receptor-γ and retinoic acid receptor-α
Variations in parametrial white adipose tissue mass during the mouse estrous cycle:: relationship with the expression of peroxisome proliferator-activated receptor-γ and retinoic acid receptor-α
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DOI：
10.1139/y06-032
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发表时间：
2006-08-01
影响因子：
2.1
通讯作者：
Murphy, Liam J.
中科院分区：
文献类型：
--
作者：
Gui, Yaoting;Cai, Zhiming;Murphy, Liam J.
雌激素和孕酮参与脂肪组织代谢的调节，过氧化物酶体增殖物激活受体-γ（PPAR γ）和视黄酸受体-α（RXR α）是脂肪组织发育所必需的。本研究旨在探讨小鼠动情周期中子宫旁组织脂肪量及PPAR γ和RXR α表达的变化。称量雌性小鼠子宫旁白色脂肪组织（WAT）、肩胛间棕色脂肪组织和子宫。采集血样用于测量17 β-雌二醇和孕酮水平。RNA酶保护试验和蛋白质印迹分析用于比较脂肪组织中PPAR γ和RXR α的表达。间情期子宫旁组织WAT的质量显著高于发情期。而棕色脂肪组织的质量在发情周期中无显著差异。间情期WAT中PPAR γ的表达明显高于动情期。在发情周期中，RXR α的表达在白色和棕色脂肪组织中均无变化。总之，子宫旁WAT质量的变化在小鼠发情周期与WAT中的PPAR γ表达的变化相关。
Estrogen and progestin participate in the regulation of adipose tissue metabolism, and peroxisome proliferator-activated receptor-gamma (PPAR gamma) and retinoic acid receptor-alpha (RXR alpha) are absolutely required for adipose tissue development. The present study is to investigate the changes in parametrial fat mass and expression of PPAR gamma and RXR alpha during estrous cycle in mice. Parametrial white adipose tissues (WAT), inter-scapula brown adipose tissues, and uteri from female mice were weighed. Blood samples were collected for the measurement of 17 beta-estradiol and progesterone levels. An RNase protection assay and Western blot analysis were used to compare the expression of PPAR gamma and RXR alpha in adipose tissue. The mass of parametrial WAT in diestrus was significantly higher compared with estrus. However, there is no significant difference on the mass of brown adipose tissues during estrous cycle. The expression of PPAR gamma in WAT in diestrus was significantly higher than that in estrus. The expression of RXR alpha during estrous cycle was unchanged in both white and brown adipose tissues. In conclusion, the variation in parametrial WAT mass during the mouse estrous cycle correlates with changes in the expression of PPAR gamma in WAT.