Overexpression of vacuolar H+-ATPase-related genes in bottom-fermenting yeast enhances ethanol tolerance and fermentation rates during high-gravity fermentation
Overexpression of vacuolar H+-ATPase-related genes in bottom-fermenting yeast enhances ethanol tolerance and fermentation rates during high-gravity fermentation
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DOI：
10.1002/jib.32
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发表时间：
2012-01-01
影响因子：
2.6
通讯作者：
Shima, Jun
中科院分区：
文献类型：
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作者：
Hasegawa, Sonoko;Ogata, Tomoo;Shima, Jun
据认为，极性W-ATP酶（V-ATP酶）在胁迫耐受中起作用。在这项研究中，它被发现，底部发酵酵母菌株，其中V-ATP酶相关基因DBF 2，VMA 41/CYS 4/NHS 5和RAV 2过表达，表现出更强的乙醇耐受性比亲本菌株，并显示出增加的发酵速率在高糖培养基模拟高浓度发酵。在所检测的菌株中，DBF 2-过表达的底部发酵酵母菌株在YPM 20培养基中表现出最高的乙醇耐受性和发酵速率。使用该菌株，通过向麦芽汁中添加糖进行高浓度发酵，与亲本菌株相比，这导致发酵速率和酵母活力增加。这些发现表明V-ATPase是高重力发酵中的应激靶标，并且表明增强V-ATPase活性增加了底部发酵酵母的乙醇耐受性，从而提高了高重力条件下的发酵速率和细胞活力。版权所有（c）2012酿酒和蒸馏研究所
Vacuolar W-ATPase (V-ATPase) is thought to play a role in stress tolerance. In this study it was found that bottom-fermenting yeast strains, in which the V-ATPase-related genes DBF2, VMA41/CYS4/NHS5 and RAV2 were overexpressed, exhibited stronger ethanol tolerance than the parent strain and showed increased fermentation rates in a high-sugar medium simulating high-gravity fermentation. Among the strains examined, the DBF2-overexpressing bottom-fermenting yeast strain exhibited the highest ethanol tolerance and fermentation rate in YPM20 medium. Using this strain, high-gravity fermentation was performed by adding sugar to the wort, which led to increased fermentation rates and yeast viability compared with the parent strain. These findings indicate that V-ATPase is a stress target in high-gravity fermentation and suggests that enhancing the V-ATPase activity increases the ethanol tolerance of bottom-fermenting yeast, thereby improving the fermentation rate and cell viability under high-gravity conditions. Copyright (c) 2012 The Institute of Brewing & Distilling