Characterization of Staphylococcus aureus coagulase type VII isolates from staphylococcal food poisoning outbreaks (1980-1995) in Tokyo, Japan, by pulsed-field gel electrophoresis
Characterization of Staphylococcus aureus coagulase type VII isolates from staphylococcal food poisoning outbreaks (1980-1995) in Tokyo, Japan, by pulsed-field gel electrophoresis
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DOI：
10.1128/jcm.38.10.3746-3749.2000
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发表时间：
2000-10-01
影响因子：
9.4
通讯作者：
Kawano, J
中科院分区：
文献类型：
--
作者：
Shimizu, A;Fujita, V;Kawano, J
金黄色葡萄球菌凝固酶Cn型菌株是日本东京葡萄球菌食物中毒暴发中最常见的菌株。我们应用脉冲场凝胶电泳（PFGE）的染色体DNA与SmaI消化，以表征129凝固酶型VN菌株。从1980-1995年35个地区129例暴发病例中分离到129株菌株。129株暴发菌株分为A型（rt = 115株）、B型（n = 10株）和C型（n = 4株）。ci型和C型又分别分为A1 ~ A33型33个和C1 ~ C4型4个。从同一地区的食物中毒病例中分离到的菌株，其亚型在1年或2年至5年之间。PFGE分型是一种有用的分型方法。金黄色葡萄球菌凝固酶VII型。在葡萄球菌性食物中毒的流行病学调查中，将凝固酶分型和PFGE分型相结合，可提供比前者更详细的信息。
Staphylococcus aureus coagulase type Cn strains have been the strains most frequently isolated from staphylococcal food poisoning outbreaks in Tokyo, Japan. We applied pulsed-field gel electrophoresis (PFGE) of chromosomal DNA digested with SmaI to characterize 129 coagulase type Vn strains. These were isolated from 129 cases occurring in outbreaks in 35 districts during a 16-year period (1980-1995), The 129 outbreak strains were classified into three types, designated A (rt = 115), B (n = 10), and C (n = 4). Types ci and C were further divided into 33 (Al to A33) and 4 (C1 to C4) subtypes, respectively. Strains of the same subtypes n cre isolated from food poisoning cases in the same districts at time intervals of 1 or 2 to 5 vears. PFGE typing appears to be a useful method for subdividing strains of S. aureus coagulase type VII. A combination of coagulase typing and PFGE typing would provide more detailed information than the former method atone in epidemiologic investigations of staphylococcal food poisoning.