Genetic improvement of heavy metal tolerance in plants by transfer of the yeast metallothionein gene (CUP1)
Genetic improvement of heavy metal tolerance in plants by transfer of the yeast metallothionein gene (CUP1)
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DOI：
10.1023/a:1004222612602
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发表时间：
1997-10-01
期刊：
影响因子：
4.9
通讯作者：
Yazaki, J
中科院分区：
文献类型：
--
作者：
Hasegawa, I;Terada, E;Yazaki, J
花椰菜（Brassica oleracea var. botrytis）耐受25 μ M CdCl2处理8天，但被50 μ M浓度的CdCl2杀死。然而，即使是15 μ M的CdCl2在1 mM的l -丁硫氨酸亚硫胺（BSO）存在下也具有毒性，这表明存在一种cd诱导的植物螯合素，并参与了花椰菜的cd耐受性。为了培育耐重金属转基因植物，我们将CaMV35S启动子下游的酵母金属硫蛋白基因（CUP1）结构基因连接到花菜中。选择了一株耐Cd转基因花椰菜，该花椰菜在400亩或更少的Cd环境下生长良好，而非转基因花椰菜仅耐受25亩的Cd。转基因花椰菜的Cd积累量高于非转基因花椰菜，特别是在上部叶片中。综上所述，通过将酵母金属硫蛋白基因转移到花椰菜中，可以提高植物的cd耐受性和cd积累能力。
Cauliflower (Brassica oleracea var. botrytis) tolerates treatment with 25 mu M CdCl2 for eight days, but is killed by that with a 50 mu M concentration. However, even 15 mu M CdCl2 is toxic in the presence of 1 mM L-buthionine sulfoximine (BSO), suggesting the presence of a Cd-inducible phytochelatin and its involvement in Cd-tolerance in cauliflower. To develop heavy metal-tolerant transgenic plants, we ligated the structural gene of yeast metallothionein gene (CUP1) downstream of CaMV35S promoter and introduced the fused gene into cauliflower. A Cd-tolerant transgenic cauliflower was selected, which grew well in the presence of 400 mu M or less Cd, whereas the non-transformed cauliflower tolerated only up to 25 mu M Cd. The transgenic cauliflower accumulated more Cd, especially in the upper leaves, than the non-transformed plant.In conclusion, by transfer of the yeast metallothionein gene into cauliflower increased Cd-tolerance and CD-accumulating ability can be conferred to the plant.