Adipose-derived stem cell transplantation for therapeutic lymphangiogenesis in a mouse secondary lymphedema model.
Adipose-derived stem cell transplantation for therapeutic lymphangiogenesis in a mouse secondary lymphedema model.
复制标题
DOI：
10.2217/rme.15.24
复制
发表时间：
2015-08
影响因子：
2.7
通讯作者：
Shuhei Yoshida;R. Hamuy;Yuuichi Hamada;H. Yoshimoto;A. Hirano;S. Akita
中科院分区：
文献类型：
--
作者：
Shuhei Yoshida;R. Hamuy;Yuuichi Hamada;H. Yoshimoto;A. Hirano;S. Akita
目的继发性淋巴水肿在乳房和妇科器官的恶性肿瘤后治疗后很常见，但尚未建立有效的治疗方法。脂肪干细胞（ADSC）具有多能性，局部注射可再生，通过再生方法测试小鼠后肢继发性淋巴水肿。方法与结果将小鼠分为四组：无ADSCs组、1×10(6)个ADSCs组、1×10(5)个ADSCs组和1×10(4)个ADSCs组（每组，n=20），并进行严格的手术切除和放射治疗。进行周长测量、淋巴流量评估和淋巴管量化。结果 LYVE-1 免疫组织化学显示，移植组中淋巴管数量以及 VEGF-C 或 VEGFR3 表达细胞显着增加（p < 0.05）。结论 ADSCs可以恢复继发性淋巴水肿伴集合管增多的淋巴管网络。
AIM Secondary lymphedema is observed in common after postmalignancy treatment of the breast and the gynecologic organs but effective therapies are not established. Adipose-derived stem cells (ADSCs), which are pluripotent, regenerative in local injection, are tested for murine hindlimb secondary lymphedema by regenerative method. METHODS & RESULTS Mice were divided into four groups: no ADSCs, 1 × 10(6) ADSCs, 1 × 10(5) ADSCs and 1 × 10(4) ADSCs (each group, n = 20) in a stringent surgical resection and irradiation. Circumferential measurement, lymphatic flow assessment and quantification of lymphatic vessels were performed. RESULTS The numbers of lymphatic vessels by LYVE-1 immunohistochemistry, and VEGF-C- or VEGFR3-expressing cells were significantly increased in transplanted groups (p < 0.05). CONCLUSION ADSCs can restore the lymphatic vascular network in secondary lymphedema with increased collecting vessels.