Desorption-ionization mass spectrometry on porous silicon
Desorption-ionization mass spectrometry on porous silicon
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DOI：
10.1038/20400
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发表时间：
1999-05-20
期刊：
影响因子：
64.8
通讯作者：
Siuzdak, G
中科院分区：
文献类型：
--
作者：
Wei, J;Buriak, JM;Siuzdak, G
自90多年前进行原始实验以来，解吸质谱法经历了重大改进（1）。最引人注目的变化发生在20世纪80年代初，引入了有机基质（2 - 4）将能量转移到分析物。这减少了离子碎裂，但也从基质引入了背景离子。在这里，我们描述了一种基于脉冲激光解吸电离的多孔硅（5）表面的生物分子质谱的无基质策略。我们的方法使用多孔硅来捕获沉积在表面上的分析物，并使用激光照射来蒸发和蒸发它们。我们表明，该方法在飞摩尔和阿托摩尔水平的分析物，并诱导很少或没有碎片，与通常观察到的其他这样的方法（6 - 11）。在没有基质的情况下进行这些测量的能力（3，4，12，13）也使其更适合小分子分析。多孔硅的化学（14）和结构（15）改性使得能够优化表面的电离特性。我们的技术提供了良好的灵敏度以及与硅基微流体和微芯片技术的兼容性。
Desorption mass spectrometry has undergone significant improvements since the original experiments were performed more than 90 years ago(1). The most dramatic change occurred in the early 1980s with the introduction of an organic matrix(2-4) to transfer energy to the analyte. This reduces ion fragmentation but also introduces background ions from the matrix. Here we describe a matrix-free strategy for biomolecular mass spectrometry based on pulsed-laser desorption-ionization from a porous silicon(5) surface. Our method uses porous silicon to trap analytes deposited on the surface, and laser irradiation to vaporize and ionize them. We show that the method works at femtomole and attomole levels of analyte, and induces little or no fragmentation, in contrast to what is typically observed with other such approaches(6-11). The ability to perform these measurements without a matrix(3,4,12,13) also makes it more amenable to small-molecule analysis. Chemical(14) and structural(15) modification of the porous silicon has enabled optimization of the ionization characteristics of the surface. Our technique offers good sensitivity as well as compatibility with silicon-based microfluidics and microchip technologies.